目的 通過(guò)薄層色譜（TLC）法、高效液相色譜（HPLC）法以及指紋圖譜的建立對(duì)強(qiáng)力腦清素片進(jìn)行質(zhì)量控制。方法 采用TLC對(duì)強(qiáng)力腦清素片中的主要組分刺五加浸膏、五味子流浸膏和鹿茸精進(jìn)行定性鑒別，以HPLC對(duì)君藥刺五加浸膏中主要有效成分紫丁香苷、刺五加苷E、異嗪皮啶同時(shí)進(jìn)行定量測(cè)定，并利用HPLC對(duì)強(qiáng)力腦清素片建立指紋圖譜。結(jié)果 強(qiáng)力腦清素片TLC色譜中的斑點(diǎn)清晰，與對(duì)照品斑點(diǎn)顏色一致。HPLC法測(cè)定強(qiáng)力腦清素片中紫丁香苷、刺五加苷E、異嗪皮啶分別在22.74～454.80、16.112～322.240、4.152～83.040 μg/mL線性關(guān)系良好，平均回收率分別為95.48%、98.22%、100.75%，質(zhì)量分?jǐn)?shù)分別為0.42～0.65、0.12～0.15、0.26～0.37 mg/片。指紋圖譜的相似度>0.95，指認(rèn)出6個(gè)共有成分，分別為刺五加浸膏中紫丁香苷、異嗪皮啶、刺五加苷E；五味子流浸膏中五味子醇甲、五味子甲素、五味子乙素。結(jié)論 TLC、HPLC結(jié)合指紋圖譜方法對(duì)強(qiáng)力腦清素片能實(shí)現(xiàn)全面的質(zhì)量控制，且操作簡(jiǎn)便、重現(xiàn)性良好。

Objective To determine the effective components of Qiangli Naoqingsu Tablets by TLC-HPLC and to study the fingerprint of the whole variety. Methods TLC of powerful brain slice of the main components in the clear element acanthopanax extract, the extract of fructus schisandrae flow and antler extract qualitative identification, by HPLC to gentleman medicine acanthopanax extract main effective component in the lilacs glycosides, acanthopanax nucleoside E, different oxazine organism under the condition of the same chromatographic determination of content, and use the HPLC to strong clear element to establish fingerprint of brain research. Results The spots in TLC were clear, aligned with those in the control and the color was consistent. The linear relationship of syringin, eleutheroside E and isofraxidin in Qiangli Naoqingsu Tablets was determined by HPLC in the range of 22.74-454.80 μg/mL, 16.112-322.240 μg/mL and 4.152-83.040 μg/mL, respectively. The average recovery rates were 95.48%, 98.22% and 100.75%, respectively, and their contents were 0.42-0.65, 0.12-0.15, 0.26-0.37 mg/tablet. Fingerprint similarity > 0.95, identified six common components, respectively, in acanthopanax extract: syringin, eleutheroside E and isofraxidin; fructus schisandrae A, fructus schisandrae A and fructus Schisandrae B in schisandrae flow extract. Conclusion TLC-HPLC and fingerprint chromatogram can control the quality of Qiangli Naoqingsu Tablets, and the operation is simple and reproducible.

R927.2