目的 基于整合文獻(xiàn)挖掘和網(wǎng)絡(luò)藥理學(xué)探究黃葵膠囊治療糖尿病腎?。―N）的作用機(jī)制。方法 通過中國學(xué)術(shù)期刊全文數(shù)據(jù)庫（CNKI）、萬方數(shù)據(jù)庫和Web of Science文獻(xiàn)檢索平臺獲取黃蜀葵花化學(xué)成分，并借助PubChem數(shù)據(jù)庫與ChemBioDraw軟件下載化合物結(jié)構(gòu)；借助Swiss Target Prediction和Stitch數(shù)據(jù)庫預(yù)測化學(xué)成分相應(yīng)靶點，并于CTD、DrugBank、GeneCards和TTD數(shù)據(jù)庫中獲得的DN靶點取交集，得到“成分-疾病”直接共有靶點；利用GeneMANIA獲取間接靶點并借助Cytoscape軟件構(gòu)建蛋白質(zhì)互作網(wǎng)絡(luò)圖；通過基因本體論和京都基因與基因組百科全書數(shù)據(jù)庫（KEGG）對潛在相關(guān)靶點進(jìn)行基因功能富集分析和通路富集分析，并構(gòu)建黃蜀葵花治療DN的“成分-直接靶點-間接靶點-通路”網(wǎng)絡(luò)圖。結(jié)果 經(jīng)文獻(xiàn)檢索得到黃葵膠囊化學(xué)成分50個，數(shù)據(jù)庫預(yù)測其治療糖尿病腎病相關(guān)靶點182個，KEGG通路富集分析得到187個相關(guān)通路，其中6個關(guān)鍵通路可以通過參與調(diào)節(jié)多個生物過程改善DN疾病的發(fā)展。結(jié)論 通過文獻(xiàn)挖掘與網(wǎng)絡(luò)藥理學(xué)初步揭示了黃葵膠囊參與調(diào)節(jié)機(jī)體糖脂代謝、氧化應(yīng)激、炎癥反應(yīng)、血流動力學(xué)紊亂和自噬等生物過程改善DN疾病的發(fā)展。

Objective To study the mechanism of Huangkui capsule in the treatment of diabetic nephropathy (DN) based on literature mining and network pharmacology. Methods The constituents of A. manihot were obtained by CNKI、Wanfang database and Web of Science, and the PubChem database and the ChemBioDraw software were used to download the structures of the compounds. Then, the corresponding targets of chemical constituents were predicted in Swiss Target Prediction and Stitch database, and intersected with the DN targets predicted in CTD, DrugBank, GeneCards and TTD databases to obtain the direct targets of "component-disease". The indirect targets were obtained through GeneMANIA, and the protein interaction network map was constructed with the help of Cytoscape software. Finally, Gene ontology and KEGG database were to conduct the Gene function enrichment analysis and pathway enrichment analysis of potential related targets, and the "component-direct target-indirect targetpathway" network diagram of A. manihot in the treatment of DN was constructed. Results Totally 50 chemical constituents of A. manihot were obtained, 182 targets related to the treatment of DN were predicted and 187 pathways were obtained by KEGG pathway analysis, of which 6 key pathways can regulate multiple biological processes to improve the development of DN. Conclusions Through literature mining and network pharmacology, this study reveals that Huangkui Capsule can participate in regulating biological processes such as glucose and lipid metabolism, oxidative stress, inflammation, hemodynamic disorders and autophagy to improve the development of DN disease, which provides a basis for further study of related mechanisms.

R285.5