目的 采用大鼠大腦中動脈阻塞/再灌注（MCAO/R）模型考察注射用丹參多酚酸（SAFI）與丁苯酞氯化鈉注射液（BSCI）聯(lián)合使用對腦缺血再灌注大鼠的治療作用。方法 構(gòu)建大鼠MCAO/R模型，術(shù)后24 h根據(jù)神經(jīng)功能缺損評分將大鼠平均分為：模型組、SAFI（11.52 mg/kg，每天給藥1次）組、BSCI（8.86 mL/kg，以丁苯酞計為2.22 mg/kg，每天給藥2次）組、SAFI（11.52 mg/kg，每天給藥1次）＋BSCI（2.22 mg/kg，每天給藥2次）組，每組20只。各組大鼠均于分組后立即尾iv給藥，連續(xù)給藥14 d。給藥體積均為10 mL/kg，假手術(shù)組和模型組給予0.9%的氯化鈉注射液?？疾旖o藥后各組大鼠神經(jīng)功能缺損評分；ELISA法檢測缺血半腦組織中白細胞介素-1β（IL-1β）、白細胞介素-6（IL-6）、腫瘤壞死因子-α（TNF-α）和超氧化物歧化酶（SOD）的含量；TTC染色檢測腦梗死面積；HE染色觀察腦組織病理變化。結(jié)果 與給藥前及模型組比較，SAFI和SAFI＋BSCI組神經(jīng)功能缺損評分顯著降低（P<0.05）；與模型組比較，3個給藥組大鼠腦組織IL-6、IL-1β和TNF-α水平均顯著降低，SOD水平顯著升高（P<0.05、0.01）； 3個給藥組的大鼠腦梗死面積均顯著減?。?i>P<0.01、0.001），其中SAFI＋BSCI組的梗死面積最小； 3個給藥組腦組織和細胞壞死的現(xiàn)象均減輕，其中SAFI和SAFI＋BSCI組表現(xiàn)出更少的炎性浸潤和瘀血現(xiàn)象。結(jié)論 SAFI、BSCI和SAFI＋BSCI均能顯著改善缺血性腦卒中大鼠的神經(jīng)損傷，SAFI和BSCI聯(lián)用有一定的藥效協(xié)同作用。

Objective To investigate the synergistic effect of Salvianolic Acid for Injection (SAFI) combined with Butylphthalide and Sodium Chloride Injection (butylphthalide) by middle cerebral artery occlusion/reperfusion (MCAO/R) model. Methods The rat MCAO/R model was established, and 24 h after surgery, the rats were divided into:model group, SAFI (11.52 mg/kg, once a day) group, BSCI (2.22 mg/kg, twice a day) group, SAFI (11.52 mg/kg, once a day) + BSCI (2.22 mg/kg, twice a day) group, 20 in each group. Rats in all groups were given tail-IV medication immediately after grouping for 14 consecutive days. The volume of administration was 10 mL/kg, and the sham operation group and model group were given 0.9% sodium chloride injection. The levels of interleukin-1 β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and superoxide dismutase (SOD) in ischemic cerebral tissue were determined by ELISA and neurological deficit score after administration. The area of cerebral infarction was detected by TTC staining. HE staining was used to observe the pathological changes of brain tissue. Results Compared with the same group before administration and model group, neurological deficit score in SAFI and SAFI + BSCI groups was significantly decreased (P<0.05). Compared with model group, the levels of IL-6, IL-1β and TNF-α in brain tissue of rats in three drug treatment groups were significantly decreased, while SOD level was significantly increased (P<0.05, 0.01). The cerebral infarction area of rats in the three drug administration groups was significantly decreased (P<0.01, 0.001), and the SAFI+BSCI group had the smallest infarct area. Tissue and cell necrosis were reduced in all three groups, and the SAFI and SAFI+BSCI groups showed less inflammatory infiltration and congestion. Conclusion SAFI, BSCI and SAFI + BSCI can significantly improve nerve injury in ischemic stroke rats. The combination of SAFI and BSCI has better efficacy.

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