目的 運用細胞膜色譜（CMC）技術檢測分析人參皂苷與五味子木脂素中15種成分與血管內(nèi)皮生長因子（VEGF）受體的作用。方法 運用Western blotting法檢測篩選VEGF受體高表達細胞株；構建VEGF受體CMC，并建立ECV304/CMC online-LC檢測方法，以索拉菲尼作為陽性藥，對人參皂苷Rb₁、Rb₂、Rb₃、Rc、Rd、Rg₁、Rg₂、Rg₃、Rh₁、Ro、F₂，五味子甲素，五味子乙素，五味子醇甲，五味子醇乙進行VEGF受體結合強度的檢測分析；CCK-8法檢測篩選出的成分（5、10 μg/mL）對ECV304細胞的促增殖作用。結果 Western blotting結果顯示，ECV304細胞中VEGF受體高表達；人參皂苷Rb₁、Rb₂、Rb₃、Rc、Rd、Rg₃、Rh₁、F₂、五味子甲素、五味子乙素與VEGF受體CMC柱有結合作用；CCK-8法檢測以上成分對ECV304細胞的活力影響發(fā)現(xiàn)，人參皂苷Rb₂能促進細胞的增殖。結論 人參皂苷Rb₂能與VEGF受體結合，且顯著促進ECV304細胞增殖。

Objective To detect and analyze the effect of 15 components from ginseng saponins and schisandra lignin on angiogenesis by cell membrane chromatography. Methods Western blotting detection was used to screen the cell lines with high expression of VEGF receptor. Cell membrane chromatographic column was prepared by ECV304 cell membrane and ECV304/CMC online-LC detection method was established. With sorafenib as a positive drug, the receptor binding strength of VEGF was detected and analyzed for ginsenosides Rb₁,Rb₂,Rb₃,Rc,Rd,Rg₁,Rg₂,Rg₃,Rh₁,Ro,F₂, deoxyschizandrin, γ -schizandrin, schisandrol A, schisandrol B. CCK-8 assay was used to detect the proliferative effects of the selected ingredients (5 and 10 μg/mL) on ECV304 cells. Results Western blotting results showed that VEGF receptor was highly expressed in ECV304 cells. It was found that ginsenoside Rb₁,Rb₂,Rb₃,Rc,Rd,Rg₃,Rh₁,F₂, schisandrin A and schisandrin B had binding effect. CCK-8 assay showed that ginsenoside Rb₂ could promote the proliferation of ECV304 cells. Conclusion Ginsenoside Rb₂ can bind to angiogenic VEGF receptor, and can significantly promote the proliferation of ECV304.

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