目的 評(píng)價(jià)注射用丹參多酚酸（SAFI）對(duì)血小板功能的影響。方法 采用大鼠體內(nèi)實(shí)驗(yàn)和家兔體外實(shí)驗(yàn)。在體內(nèi)實(shí)驗(yàn)中，40只大腦缺血-再灌注模型（MCAO/IR）大鼠隨機(jī)分為模型組，SAFI低、中、高劑量（5.8、11.6、23.2 mg/kg）組和陽性藥（銀杏內(nèi)酯注射液，2 mL/kg）組，另取8只切開頸部皮膚作為假手術(shù)組。尾靜脈iv給藥14 d后分別取血，凝血時(shí)間檢測試劑盒檢測活化凝血時(shí)間（ACT）和活化部分凝血活酶時(shí)間（APTT），利用ELISA試劑盒測定各組血清中5-羥色胺（5-HT）、P選擇素（CD62P）、β球蛋白（β-TG）和血小板膜糖蛋白Ⅱa/Ⅲb（GPⅡa/Ⅲb）含量。體外實(shí)驗(yàn)中，采用腺苷二磷酸（ADP），血小板活化因子（PAF）和花生四烯酸（AA）為誘導(dǎo)劑，誘導(dǎo)家兔血小板聚集，通過血小板分析儀測定不同濃度的SAFI對(duì)血小板聚集率的影響。結(jié)果 體內(nèi)實(shí)驗(yàn)結(jié)果表明：低、中、高劑量SAFI均能明顯延長大鼠血液凝血時(shí)間，且能有效降低模型大鼠血液中5-HT、CD62p、β-TG和GPⅡb/Ⅲa的水平（P<0.05、0.01）。體外實(shí)驗(yàn)結(jié)果表明：SAFI均可顯著降低血小板聚集率、抑制血小板聚集（P<0.05、0.01）。結(jié)論 SAFI可有效抑制血小板活化，改善血小板的黏附、聚集、釋放等功能。

Objectives To explore the effect of Salvianolic Acids for Injection (SAFI) on platelet function. Methods The effects were evaluateed by rats experiment in vivo and rabbits experiment in vitro. In vivo experiments, cerebral ischemia-reperfusion model (MCAO/IR) rats were constructed by carotid artery embolization, and 40 rats that were successfully modeled were randomly divided into model group, SAFI low-dose group (5.8 mg/kg), medium-dose group (11.6 mg/kg) and high-dose group (23.2 mg/kg), and positive drug group (Ginkgolides Injection, 2 mL/kg). Another eight rats were cut into the neck skin without modeling as the sham operation group. Blood samples were collected after 14 days of caudal vein administration. Activated coagulation time (ACT) and activated partial thrombin time (APTT) were measured using a clotting time detection kit. The contents of 5-HT, CD62P, β-TG and GP Ⅱ a/Ⅲ b in serum of each group were determined by ELISA kit. In vitro experiment, adenosine diphosphate (ADP), platelet activating factor (PAF) and arachidonic acid (AA) were used as inducers to induce platelet aggregation in rabbits. Results The results of in vivo experiments showed that low, medium and high dose SAFI can significantly prolong the blood coagulation time of rats, and effectively reduced the levels of 5-HT, CD62p, β-TG and GPⅡa/Ⅲb in blood of model rats (P<0.05, 0.01). The results of in vitro experiments showed that SAFI could significantly reduce the platelet aggregation rate and inhibit platelet aggregation in ADP, PAF and AA induced platelet aggregation (P<0.05, 0.01). Conclusions SAFI can effectively inhibit platelet activation and improve platelet adhesion, aggregation and release.

R285.5