目的 研究白鮮堿對(duì)皮膚淺部真菌紅色毛癬菌的體外抑菌作用。方法 采用液基稀釋法測(cè)定白鮮堿對(duì)紅色毛癬菌的最低抑菌濃度（MIC）；采用高通量測(cè)序技術(shù)對(duì)白鮮堿（25.0、12.5 μg/mL）作用的紅色毛癬菌進(jìn)行轉(zhuǎn)錄組測(cè)序，對(duì)測(cè)序序列進(jìn)行處理和生物學(xué)信息分析，進(jìn)行差異表達(dá)基因、基因本體論（GO）功能顯著性和京都基因與基因組百科全書（KEGG）富集分析等。結(jié)果 液基稀釋法結(jié)果表明，白鮮堿對(duì)紅色毛癬菌的MIC為50 μg/mL。高通量測(cè)序結(jié)果顯示，對(duì)照組與白鮮堿 25.0 μg/mL組比較，差異基因總數(shù)為890個(gè)；其中上調(diào)表達(dá)384個(gè)，下調(diào)表達(dá)542個(gè)。白鮮堿作用后紅色毛癬菌的差異基因主要表現(xiàn)于細(xì)胞膜結(jié)構(gòu)、膜固有成分、氧化還原酶活性、對(duì)藥物的反應(yīng)和細(xì)胞色素復(fù)合體等。白鮮堿作用后紅色毛癬菌在代謝途徑、MAPK信號(hào)通路、ABC轉(zhuǎn)運(yùn)蛋白合成、色氨酸代謝等富集通路相關(guān)基因均有顯著性改變（P<0.05）。結(jié)論 白鮮堿對(duì)紅色毛癬菌的抑菌機(jī)制可能與抑制細(xì)胞膜結(jié)構(gòu)的完整性、影響紅色毛癬菌的能量代謝、降低耐藥性等途徑有關(guān)。

Objective To study the antibacterial effect of dictamnine on Trichophyton rubrum. Methods The minimal inhibitory concentration (MIC) of dictamnine against Trichophyton rubrum was determined by liquid based dilution method. The transcriptome of Trichophyton rubrum treated with dictamnine was sequenced by high-throughput sequencing technology, and the sequencing sequence was processed and the biological information was analyzed to analyze the differentially expressed genes, the significance of GO function and KEGG metabolic pathway. Results the MIC of dictamnine against Trichophyton rubrum was 50 μg/mL. The results of high-throughput sequencing showed that there were 890 differentially expressed genes in the control group compared with the high-dose group, including 384 up-regulated genes and 542 down regulated genes. The differential genes of Trichophyton rubrum treated with dictamnine were mainly expressed in cell membrane structure, membrane intrinsic components, oxidoreductase activity, drug response and cytochrome complex. There were significant changes in metabolic pathway, MAPK signaling pathway, ABC transporter synthesis and tryptophan metabolism of Trichophyton rubrum after dictamnine treatment (P<0.05). Conclusion The antibacterial mechanism of dictamnine against Trichophyton rubrum may be related to inhibiting the integrity of cell membrane structure, affecting the energy metabolism of Trichophyton rubrum and reducing drug resistance.

R285.5

內(nèi)蒙古自治區(qū)科技計(jì)劃項(xiàng)目（201802117）