目的 評(píng)價(jià)高效液相色譜-串聯(lián)質(zhì)譜（LC-MS/MS）法檢測(cè)人血漿依托考昔濃度的不確定度。方法 采用所建立數(shù)學(xué)模型對(duì)影響人血漿依托考昔濃度測(cè)定的因素——重復(fù)性、稱量、溶液配制、標(biāo)樣配制、樣品提取、基質(zhì)效應(yīng)、線性擬合、儀器量化等，進(jìn)行量化、合成及擴(kuò)展。結(jié)果 置信概率（P）為95%時(shí)，依托考昔低、中、高3質(zhì)量濃度可分別表示為（33.30±8.61）、（322.0±18.3）、（2 507±140）ng/mL。結(jié)論 人血漿依托考昔濃度測(cè)定方法不確定度主要來(lái)自線性擬合、溶液配制和基質(zhì)效應(yīng)。

Objective To evaluate the uncertainty of a new method for the determination of etoricoxib in human plasma. Methods The mathematical model was established, and used to quantify, synthesize and expand the influence factors including precision, weighing, solution preparation, drug-spiked plasma preparation, extraction process, matrix effect, linear fitting and equipment factor in the whole process of determination of etoricoxib in human plasma by the mathematical model. Results When the confidence probability was 95%, the low, medium and high concentrations of etoricoxib in human plasma could be expressed as (33.3±8.61), (322±18.3) and (2 507±140) ng/mL. Conclusion The uncertainty of new method mainly comes from linear fitting, solution preparation and matrix effect.

R917

山東省重點(diǎn)研發(fā)計(jì)劃（2018GSF118130）