目的 研究普通工藝、傳統(tǒng)工藝制備紫雪散的抗驚厥作用，初步探討其作用機制。方法 采用雄性SPF級ICR小鼠，隨機分為對照組，模型組，傳統(tǒng)工藝紫雪散和普通工藝紫雪散低、中、高劑量（0.78、1.56、3.12 g·kg^-1）組及5%苯巴比妥（0.01 mL·g^-1）組。通過頸背部sc尼可剎米注射液誘發(fā)小鼠驚厥模型，觀察各組小鼠治療后的驚厥潛伏期、死亡潛伏期、20 min死亡率；采用比色法檢測大腦皮層中鈣水平；Griess reagent法檢測大腦皮層總NO含量；參照試劑盒說明書檢測大腦皮層中谷氨酸（Glu）、γ-氨基丁酸（GABA）含量。結果 與模型組比較，苯巴比妥鈉、普通和傳統(tǒng)工藝紫雪散的中、高劑量均可顯著延長小鼠的驚厥潛伏期（P<0.001）；普通和傳統(tǒng)工藝紫雪散的低、中、高劑量均可顯著延長驚厥小鼠的死亡潛伏期（P<0.01、0.001）；模型組小鼠死亡率為100%，苯巴比妥鈉組沒有小鼠死亡，普通和傳統(tǒng)工藝紫雪散的中、高劑量組死亡率明顯降低。2種工藝紫雪散的抗驚厥作用具有劑量相關性，與普通工藝高劑量比較，傳統(tǒng)工藝紫雪散高劑量可以顯著延長驚厥模型小鼠的驚厥、死亡潛伏期（P<0.05、0.01），降低驚厥致死率。與模型組比較，3.12 g·kg^-1傳統(tǒng)工藝和普通工藝紫雪散、苯巴比妥鈉均可以顯著降低小鼠腦內鈣含量（P<0.05、0.001）和總NO含量（P<0.001）；與普通工藝紫雪散組比較，3.12 g·kg^-1傳統(tǒng)工藝紫雪散可以顯著降低驚厥小鼠腦內總NO的含量（P<0.01）。與模型組比較，傳統(tǒng)工藝和普通工藝紫雪散3.12 g·kg^-1組、苯巴比妥鈉組小鼠腦皮層中Glu含量顯著減少（P<0.05、0.001）；傳統(tǒng)工藝紫雪散3.12 g·kg^-1組小鼠腦皮層中GABA含量顯著增加（P<0.05）。結論 傳統(tǒng)工藝和普通工藝紫雪散均具有抗驚厥作用，并且傳統(tǒng)工藝紫雪散的抗驚厥作用優(yōu)于普通工藝紫雪散。

Objective To investigate the anticonvulsant effect of Zixue Powder and its mechanism. Methods Male SPF ICR mice were assigned to the normal group, model (nikosamide, 0.75 g·kg^-1) group, Zixue Powder traditional processes low, medium and high-dose (0.78, 1.56, 3.12 g·kg^-1) group, Zixue Powder ordinary processes low-dose, medium-dose and high-dose (0.78, 1.56, 3.12 g·kg^-1) groups, phenobarbital (0.01 mL·g^-1) groups. Model mice was injected subcutaneously through the back of the neck of nikethamide. The recorded data was the convulsion latency, death latency and 20 min mortality. The contents of calcium, total nitric oxide (NO), glutamate (Glu), and γ -aminobutyric acid (GABA) in cerebral cortex were detected. Results Compared with model group, phenobarbital sodium, Zixue Powder ordinary and traditional processes of medium and high dose can significantly prolong the incubation period of convulsion mice (P < 0.001). Low, medium and high doses of Zixue Powder could significantly prolong the incubation period of death in mice with convulsion (P < 0.01, 0.001). The mortality of mice in model group was 100%, and no mice in the phenobarbital group died. The mortality of mice in the medium and high dose groups of Zixue Powder ordinary and traditional processes was significantly reduced. The anti-convulsion effect of Zixue Powder was dose-dependent. Compared with the high dose of ordinary processes, the high dose of traditional processes could significantly prolong the incubation period of convulsion and death in mice with convulsion model (P < 0.05, 0.01), and reduce the mortality rate of convulsion. Compared with model group, phenobarbital sodium and of traditional and ordinary processes of 3.12 g·kg^-1 could significantly reduce calcium content (P < 0.05, 0.001) and total NO content (P < 0.001) in brain of mice. Compared with Zixue Powder ordinary processes group, 3.12 g·kg^-1 Zixue Powder traditional processes could significantly reduce the content of total NO in the brain of convulsive mice (P < 0.01). Compared with model group, the Glu content of Zixue Powder ordinary and traditional processes of 3.12 g·kg^-1, phenobarbital sodium group was significantly decreased (P < 0.05, 0.001). The content of GABA in cerebral cortex of Zixue Powder traditional processes was significantly increased (P < 0.05). Conclusion Both traditional and ordinary processes of Zixue Powder have anticonvulsant effect. The anticonvulsant effect of traditional processes is better than that of ordinary processes.

R285.5

“十三五”期間天津市高等學?！皠?chuàng)新團隊培養(yǎng)計劃”資助項目（TD13-5050）