發(fā)育毒性是毒理學(xué)研究中最具挑戰(zhàn)性的領(lǐng)域之一，傳統(tǒng)的發(fā)育毒性評(píng)價(jià)方法均存在實(shí)驗(yàn)成本高、周期長(zhǎng)、技術(shù)復(fù)雜等弊端。人多能干細(xì)胞（hPSC）包括人胚胎干細(xì)胞（hESC）和誘導(dǎo)多能干細(xì)胞（iPSC），具有分化多能性和無(wú)限增殖的潛力。近年來hPSC在發(fā)育毒性評(píng)價(jià)方面的研究逐漸增多，主要從hPSCs培養(yǎng)中代謝小分子的變化、hPSCs的定向分化、擬胚體的形態(tài)結(jié)構(gòu)和轉(zhuǎn)錄組學(xué)變化、3D器官培養(yǎng)等方面綜述了基于hPSC的藥物發(fā)育毒性評(píng)價(jià)的最新研究進(jìn)展，進(jìn)而為今后藥物發(fā)育毒性的研究提供參考。

Developmental toxicity is one of the most challenging fields in toxicology. The traditional developmental toxicity evaluation methods have the disadvantages of high cost, long cycle and complex technology. Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSC), have the potential of pluripotent differentiation and unlimited proliferation. In recent years, there are more researches on hPSCs in developmental toxicity evaluation. This paper mainly reviews the latest research progress of hPSCs based developmental toxicity evaluation from the aspects of the changes of small metabolic molecules in hPSCs culture, the induced differentiation of hPSCs, the morphological structure and transcriptome changes of embryoid, and 3D organ culture, then provides reference methods for the study of drug developmental toxicity.

R965.3

國(guó)家自然科學(xué)基金資助項(xiàng)目（82074275）