目的 探討千金黃連方對(duì)2型糖尿病模型動(dòng)物db/db小鼠血糖的影響。方法 C57BL/6J小鼠10只（雌雄各半）作為對(duì)照組，將db/db小鼠60只（雌雄各半）按血糖值隨機(jī)分為6組：模型組、二甲雙胍（0.25 g·kg^-1）組、金芪降糖片（4.2 g·kg^-1）組和千金黃連方高、中、低劑量（生藥13.00、6.50、3.25 g·kg^-1）組。每天ig給藥1次，給藥體積20 mL·kg^-1，對(duì)照組及模型組ig給予等量去離子水。血糖儀測(cè)定千金黃連方給藥1次對(duì)糖耐量、空腹血糖的影響及多次給藥對(duì)空腹血糖、糖耐量、混合飲食餐后血糖、淀粉耐量（碳水化合物飲食餐后血糖）的影響；連續(xù)給藥12周后，試劑盒法檢測(cè)血清中糖化血清蛋白（GSP）、糖化血紅蛋白（GHb）、胰島素水平；小鼠放血處死后，剪開腹部，取腹部脂肪稱質(zhì)量，計(jì)算脂肪系數(shù)；HE染色后對(duì)肝組織進(jìn)行病理學(xué)檢查。結(jié)果 給藥1次，與模型組比較，給藥后1~2 h千金黃連方高劑量能顯著降低小鼠空腹血糖值和糖耐量血糖值（P<0.05、0.01）。與模型組比較，多次給予千金黃連方能顯著降低小鼠空腹血糖值、顯著降低葡萄糖耐量血糖值、顯著降低混合飲食餐后血糖值和淀粉耐量血糖值（P<0.05、0.01、0.01）。與模型組比較，千金黃連方高、中、低劑量組小鼠GHb、GSP及胰島素水平均顯著下降（P<0.05、0.01、0.001）；高、中劑量組小鼠腹部脂肪系數(shù)顯著下降（P<0.05、0.01）；高、低劑量對(duì)肝臟空泡變性程度有減輕作用。結(jié)論 千金黃連方能顯著降低db/db小鼠空腹血糖，改善糖耐量異常及增強(qiáng)胰島素敏感性，顯著減少腹部脂肪系數(shù)，對(duì)肝細(xì)胞的損傷也有一定的改善作用；其降血糖作用與二甲雙胍相當(dāng)，強(qiáng)于金芪降糖片，其改善胰島素抵抗、增加胰島素敏感性的作用強(qiáng)于二甲雙胍和金芪降糖片。

Objective To investigate the effects of Qianjin Huanglian Prescription on blood glucose in db/db mouse of type 2 diabetes model. Methods Sixty db/db mice aged 4-6 weeks (half male and half female) were selected as the experimental group. Ten C57BL/6J mice aged 4-6 weeks (half male and half female) were selected as the control group. The db/db mice were randomly divided into six groups according to the blood glucose level, which were the model group, the metformin group, the Jinqi Jiangtang Tablets group, and the Qianjin Huanglian Prescription (raw medicine 13.00, 6.50, 3.25 g/kg). The effects of Qianjin Huanglian Prescription dosing once on glucose tolerance, fasting blood and The effects of Qianjin Huanglian Prescription multiple dosing on fasting blood glucose, glucose tolerance, mixed diet postprandial blood glucose, starch tolerance (carbohydrate diet postprandial blood glucose), serum glycated hemoglobin, glycosylated serum protein, insulin, abdominal fat weight and abdominal fat coefficient were measured separately.Pathological examination of liver tissues was also made. Results Dosing once, compared with the model group, the high dose of Qianjin Huanglian Prescription after 1-2 h after drug can significantly reduce the fasting blood glucose level and glucose tolerance of mice (P < 0.05 and 0.01). Multiple administration of Qianjin Huanglian Prescription can significantly reduce fasting blood glucose, improve glucose tolerance, improve Mixed diet postprandial blood glucose levels and carbohydrate diet postprandial blood glucose levels in mixed diets (P < 0.05, 0.01 and 0.001). Compared with model group, Qianjin Huanglian Prescription significantly reduce GHb and GSP levels, significantly reduce insulin level (P < 0.05, 0.01 and 0.001), improve insulin resistance; significantly reduce abdominal fat weight and abdominal fat coefficient in diabetic mice(P < 0.05 and 0.01), reduce the degree of Hepatic vacuolar degeneration. Conclusion Qianjin Huanglian Prescription can significantly reduce fasting blood glucose in db/db mice, improve impaired glucose tolerance and enhance insulin sensitivity, improve insulin resistance, and significantly reduce abdominal fat coefficient, There is also a certain improvement in the damage of hepatocytes in db/db mice. Its hypoglycemic effect is similar to metformin and stronger than Jinqi Jiangtang Tablets. Its effect of improving insulin resistance and increasing insulin sensitivity is stronger than that of metformin and Jinqi Jiangtang Tablets.

R285.5

天津市科技支撐計(jì)劃重點(diǎn)項(xiàng)目（16YFZCSY00620）