介紹了幾種常見(jiàn)的血腦屏障體內(nèi)外模型及一種腦滲透性分類方案。其中體外模型主要有溶劑水/分配模型、平行人工膜滲透模型、Transwell細(xì)胞模型、微流控芯片血腦屏障模型、永生化內(nèi)皮細(xì)胞系建立的血腦屏障模型、三維血腦屏障模型等。體內(nèi)模型主要有腦/血漿比率測(cè)定法、小鼠腦攝取量分析法、嚙齒類動(dòng)物原位腦灌注法、腦微透析法等。隨著血腦屏障模型的不斷成熟完善，將有助于篩選中樞神經(jīng)系統(tǒng)藥物，為神經(jīng)系統(tǒng)疾病的治療提供更多的理論依據(jù)。通過(guò)分析和評(píng)價(jià)各種不同的血腦屏障模型，以期為血腦屏障模型研究及中樞神經(jīng)系統(tǒng)藥物研發(fā)提供新的思路。

This article introduces several common in vivo and in vitro models of the blood-brain barrier and a classification scheme for brain permeability. Among them, in vitro models mainly include solvent water/distribution model, parallel artificial membrane permeation model, Transwell cell model, microfluidic chip blood-brain barrier model, blood-brain barrier model established by immortalized endothelial cell line, 3D blood-brain barrier model, etc. In vivo models mainly include brain/plasma ratio determination method, mouse brain uptake analysis method, rodent in situ brain perfusion method, brain microdialysis method and so on. As the blood-brain barrier model continues to mature and improve, it will help to screen drugs for the central nervous system and provide more theoretical basis for the treatment of neurological diseases. This article analyzes and evaluates various blood-brain barrier models, hoping to provide new ideas for blood-brain barrier model research and central nervous system drug research and development.

R965.3

國(guó)家重點(diǎn)研發(fā)計(jì)劃——重大疾病治療藥物制劑質(zhì)量研究評(píng)價(jià)技術(shù)聯(lián)合研究（2020YFE0201700）