排斥反應(yīng)是影響異體器官移植術(shù)后移植器官長期存活的獨立危險因素，免疫抑制劑的應(yīng)用可預(yù)防排斥反應(yīng)發(fā)生，極大地提高了患者移植物的存活率，但臨床上在使用免疫抑制劑過程中，需要對治療藥物進行濃度監(jiān)測。目前常用的檢測方法有免疫分析法及色譜法，其中液相色譜串聯(lián)質(zhì)譜法因具有專屬性強、準確度高、分析快速、靈敏度高等優(yōu)點，大多數(shù)臨床實驗室以該測定方法作為治療藥物監(jiān)測的金標準?；谂R床常用免疫抑制劑的藥理及藥動學(xué)特性，將免疫抑制劑的檢測方法分為全血型免疫抑制劑的檢測與血漿型免疫抑制劑的檢測，并分別對兩類藥物的血藥濃度監(jiān)測方法進行綜述，旨在為免疫抑制劑在器官移植領(lǐng)域的臨床安全合理用藥提供更直接的指導(dǎo)依據(jù)。

Rejection is an independent risk factor affecting the long-term survival of transplanted organs after allogeneic organ transplantation. The application of immunosuppressants can prevent rejection and greatly improve the survival rate of patient transplants. At present, the commonly used detection methods for the detection of immunosuppressants include immunoassay and chromatography. Liquid chromatography tandem mass spectrometry has the advantages of strong specificity, high accuracy, fast analysis, and high sensitivity, and is the "gold standard" in most clinical laboratories for therapeutic drug monitoring of immunosuppressants. Based on the pharmacological and pharmacokinetic characteristics of immunosuppressants commonly used in clinical practice, the detection methods of immunosuppressants are divided into immunosuppressant in blood and immunosuppressant in plasma, and the monitoring methods are reviewed, aiming to provide more significant and direct guidance for immunosuppressants clinical rational drug use application in organ transplantation.

R976

福州市科技計劃項目（2021-S-104）；湖南省重點研發(fā)計劃（2019SK2241）； 2019年度湖南創(chuàng)新型省份建設(shè)專項創(chuàng)新平臺（2019CB1014）；復(fù)雜基質(zhì)樣本生物分析湖南省重點實驗室項目（2017TP1037）；福建省衛(wèi)生健康青年科研課題（2020QNA074）