目的 基于灰色關(guān)聯(lián)度法和偏最小二乘法研究芩蘇膠囊抑菌作用的譜效關(guān)系。方法 使用高效液相色譜(HPLC)法建立10批芩蘇膠囊的指紋圖譜,采用管碟法測(cè)定芩蘇膠囊(0.3 g·mL^-1)對(duì)5種常見致病菌(金黃色葡萄球菌、大腸埃希菌、銅綠假單胞菌、肺炎克雷伯菌、肺炎鏈球菌)的抑菌活性,使用灰色關(guān)聯(lián)度分析法結(jié)合偏最小二乘法構(gòu)建譜效關(guān)系。結(jié)果 10批樣品指紋圖譜中有37個(gè)共有峰,芩蘇膠囊對(duì)5種致病菌均具有一定的抑菌作用,對(duì)金黃色葡萄球菌有較好的抑菌作用。6、10(迷迭香酸)、14、17(丹酚酸B)號(hào)峰為芩蘇膠囊抑制金黃色葡萄球菌的主要成分;10(迷迭香酸)、17(丹酚酸B)、35(苦參堿)、37(氧化苦參堿)號(hào)峰為抑制大腸埃希菌的主要成分;6、7、8、9、10(迷迭香酸)、14、17(丹酚酸B)、27、31(丹參酮Ⅰ)、37(氧化苦參堿)號(hào)峰為抑制銅綠假單胞菌的主要成分;8、9、10(迷迭香酸)、14、17(丹酚酸B)、37(氧化苦參堿)為抑制肺炎克雷伯菌的主要成分;5、23(漢黃芩素)、27、30、31(丹參酮Ⅰ)、32、33(丹參酮Ⅱ_A)、34(槐果堿)、36(氧化槐定堿)、37(氧化苦參堿)號(hào)峰為抑制肺炎鏈球菌的主要成分。結(jié)論 通過譜效關(guān)系研究明確了芩蘇膠囊抑菌藥效作用是多種成分共同作用的結(jié)果,可為芩蘇膠囊的藥效物質(zhì)基礎(chǔ)研究與質(zhì)量控制提供參考。

Objective To study the spectral effect relationship of antibacterial activity in Qinsu capsule based on grey correlation degree method and partial least square method. Methods Using HPLC to establish 10 Qinsu capsule of fingerprint, tube plate method is used to determine Qinsu capsule on five kinds of pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae) bacteriostatic activity, using grey correlation analysis method combined with partial least squares method to construct spectrum efficiency relations. Results There were 37 common peaks in the fingerprints of 10 batches of samples. Qinsu capsule had certain bacteriostatic effect on five kinds of pathogenic bacteria, and had better bacteriostatic effect on Staphylococcus aureus. Peak 6, 10 (rosinic acid), peak 14, peak 17 (salvianolic acid B) were the main components of Qinsu capsule against Staphylococcus aureus. Peak 10 (rosinic acid), peak 17 (salvianolic acid B), peak 35 (matrine), peak 37 (oxymatrine) were the main components of Qinsu capsule against Escherichia coli. Peaks 6, 7, 8, 9, 10 (rosinic acid), 14, 17 (salvianolic acid B), 27, 31 (tanshinone Ⅰ), 37 (oxymatrine) were the main components of Qinsu capsule against Pseudomonas aeruginosa. Peak 8, 9, 10 (rosinic acid), 14, 17 (salvianolic acid B), 37 (oxymatrine) were the main components of Qinsu capsule against Klebsiella pneumoniae. Peak 5, 23 (hanbaicalein), 27, 30, 31 (tanshinone Ⅰ), 32, 33 (tanshinone Ⅱ_A), 34 (sophorine), 36 (sophorine oxide), 37 (oxymatrine oxide) were the main components of Qinsu capsule against Streptococcus pneumoniae. Conclusion Through the study of spectrum effect relationship, it is clear that the antibacterial effect of Qinsu Capsule is the result of the joint action of multiple components, and this study can provide reference for the basic research and quality control of qinsu capsule.

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上海市科學(xué)技術(shù)委員會(huì)科研計(jì)劃項(xiàng)目(19401901700)