目的 建立定量檢測地膚子中9種成分含量的高效液相色譜（HPLC）法，并聯(lián)合化學(xué)計(jì)量學(xué)及灰色關(guān)聯(lián)度分析對(duì)其質(zhì)量控制進(jìn)行研究。方法 采用 HPLC法同時(shí)檢測地膚子中蘆丁、金絲桃苷、異鼠李素-3-O-葡萄糖苷、槲皮素、異鼠李素、齊墩果酸、胡蘿卜苷、地膚子皂苷Ⅰc、β-谷甾醇含量，并通過聚類分析、主成分分析、正交偏最小二乘法-判別分析及灰色關(guān)聯(lián)度分析比較不同產(chǎn)地地膚子質(zhì)量差異。結(jié)果 蘆丁、金絲桃苷、異鼠李素-3-O-葡萄糖苷、槲皮素、異鼠李素、齊墩果酸、胡蘿卜苷、地膚子皂苷Ⅰc和β-谷甾醇分別在各自范圍內(nèi)線性關(guān)系良好（r＞0.999 2）；精密度、穩(wěn)定性及重復(fù)性良好；平均加樣回收率在96.93%～100.12%。聚類分析結(jié)果顯示17批樣品聚為3類；主成分分析結(jié)果顯示主成分1、2是影響樣品質(zhì)量評(píng)價(jià)的主要因子；正交偏最小二乘法-判別分析結(jié)果顯示地膚子皂苷Ⅰc、蘆丁和齊墩果酸變量重要性投影（VIP）值均＞1，是影響產(chǎn)品質(zhì)量差異的標(biāo)志性成分；灰色關(guān)聯(lián)度分析結(jié)果顯示17批樣品的相對(duì)關(guān)聯(lián)度在0.334 0～0.622 5，不同產(chǎn)地地膚子質(zhì)量存在一定差異。結(jié)論 所建立的HPLC多組分定量聯(lián)合化學(xué)計(jì)量學(xué)及灰色關(guān)聯(lián)度分析方法操作便捷、結(jié)果準(zhǔn)確，可為地膚子質(zhì)量控制和品質(zhì)評(píng)價(jià)提供依據(jù)。

Objective To establish an HPLC method for simultaneous determination of nine components in Kochia scoparia, and to study the quality by combining chemometrics and grey relational analysis (GRA). Method HPLC method was used to simultaneously determine the contents of rutoside, hyperoside, isorhamnetin-3-O-beta-D-glucoside, quercetin, isorhamnetin, oleanolic acid, eleutheroside A, kochioside Ic and β-sitosterol in K. scoparia. The quality differences of K. scoparia from different producing areas were compared by cluster analysis, principal component analysis, orthogonal partial least-squares methoddiscriminant analysis and grey correlation analysis. Results The linear relationship of rutoside, hyperoside, isorhamnetin-3-O-beta-D-glucoside, quercetin, isorhamnetin, oleanolic acid, eleutheroside A, kochioside Ic and β -sitosterol was good in their respective ranges (r＞0.999 2). The method had good precision, stability, and repeatability (RSD＜2.0%). The average recovery (n=9) was between 96.93% and 100.12% (RSD＜2.0%). Cluster analysis showed that 17 batches of K. scoparia were clustered into three categories. Principal component analysis showed that principal component 1 and 2 was the main factor affecting the quality evaluation of the samples. Orthogonal partial least squares-discriminant analysis showed that VIP values of kochioside Ic, rutoside and oleanolic acid were all greater than 1, which were the symbolic components affecting the quality difference of products. GRA showed that the relative correlation degree of 17 batches of K. scoparia was between 0.334 0 and 0.622 5. There were some differences in the quality of K. scoparia from different producing areas. Conclusion The established HPLC multi-component quantitative method combined with chemometrics and grey relational analysis is convenient and accurate,which can provide a basis for quality control and quality evaluation of K. scoparia.

R284.1

國家自然科學(xué)基金青年科學(xué)基金項(xiàng)目（81703357）；河南省醫(yī)學(xué)科技攻關(guān)計(jì)劃聯(lián)合共建項(xiàng)目（LHGJ20210992）