目的 探討辣木葉水提物（MOLAE）對脂肪堆積人HepG2細胞的調(diào)節(jié)作用。方法 制備MOLAE凍干粉；通過CCK-8法檢測油酸鈉-鈉棕櫚酸酯（O-P）對HepG2細胞活力的影響、油紅O染色檢測O-P對細胞脂質(zhì)堆積的影響、試劑盒法檢測O-P對細胞三酰甘油（TG）、總膽固醇（TC）水平的影響，篩選O-P誘導(dǎo)HepG2細胞脂質(zhì)代謝異常模型的作用濃度及時間；CCK-8法檢測辛伐他汀、MOLAE對HepG2細胞活力的影響，篩選安全作用濃度；設(shè)立對照組、模型組、辛伐他?。栃运?，15 μmol·L^-1）組和MOLAE（3.125、6.250、12.500、25.000、50.000 μg·mL^-1）組，除對照組外，其他各組均給予0.4-0.2 mmol·L^-1的O-P誘導(dǎo)細胞脂質(zhì)沉積，誘導(dǎo)3 h后開始給藥，干預(yù)24 h后，CCK-8法檢測細胞活性，油紅O染色觀察細胞中脂滴形成情況，試劑盒法測定細胞中TG、TC、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）及丙二醛（MDA）水平。結(jié)果 確定造模方式為：0.4-0.2 mmol·L^-1的O-P作用HepG2細胞3 h后開始給藥；10、15 μmol·L^-1的辛伐他汀和3.125～100.000 μg·mL^-1的MOLAE作用于HepG2細胞24、48 h后，不影響細胞活力。與模型組比較，不同濃度的MOLAE（3.125、6.250、12.500、25.000、50.000 μg·mL^-1）均能降低TG、TC、MDA水平（P<0.05、0.01），顯著升高GSH、SOD水平（P<0.05、0.01）。油紅O染色結(jié)果表明，辛伐他汀組和MOLAE組（12.5、25.0 μg·mL^-1）脂滴堆積現(xiàn)象均較模型組有明顯改善。結(jié)論 MOLAE能夠降低O-P誘導(dǎo)的HepG2細胞中TG、TC水平，提高GSH、SOD水平和降低MDA水平，減少細胞中脂質(zhì)堆積的現(xiàn)象。

Objective To investigate the regulating effects of Moringa oleifera leaf aqueous extract (MOLAE) on lipid abnormality in human HepG2 cells. Methods Detect the effect of sodium oleate-sodium palmitate (O-P) on HepG2 cell viability using the CCK-8 method, the effect of O-P on cell lipid accumulation using oil red O staining, and the effect of O-P on cell triglyceride (TG) and total cholesterol (TC) levels using the kit method. Screen the concentration and time of O-P induced abnormal lipid metabolism model in HepG2 cells. The CCK-8 method was used to detect the effects of simvastatin and MOLAE on the viability of HepG2 cells, and to screen for safe concentrations of action. Establish control group, model group, and simvastatin (positive drug, 15 μmol·L^-1) group and the MOLAE (3.125, 6.250, 12.500, 25.000, 50.000 μg·L^-1) group, except for the control group, were all given O-P of 0.4- 0.2 mmol·L^-1 to induce cell lipid deposition. After induction for 3 h, the drug was administered. After 24 hours of intervention, cell activity was detected using CCK-8 method, lipid droplet formation was observed using oil red O staining, and the levels of TG, TC, superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH) in cells were measured using a kit method. Results The modeling method was determined as follows: Administration of O-P with a concentration of 0.4-0.2 mmol·L^-1 was initiated on HepG2 cells for 3 h. Simvastatin with 10 and 15 μmol·L^-1 and MOLAE of 3.125—100.000 μg·mL^-1 did not affect cell viability after 24 and 48 hours of action on HepG2 cells. Compared with the model group, different concentrations of MOLAE (3.125, 6.250, 12.500, 25.000, 50.000 μg·mL^-1) can reduce the levels of TG, TC, and MDA (P< 0.05, 0.01), and significantly increase the levels of GSH and SOD (P< 0.05, 0.01). The oil red O staining results showed that the accumulation of lipid droplets in the simvastatin group and MOLAE (12.5, 25.0 μg·mL^-1) group was significantly improved compared with model group. Conclusion MOLAE can reduce TG and TC levels in model group, increase GSH and SOD levels and reduce MDA levels, reduce lipid accumulation in cells and prevent lipid damage.

廣東省教育廳普通高校認定類科研項目（2020KTSCX342）；深圳市藥學(xué)會醫(yī)院藥學(xué)研究基金（恒瑞基金）（SZ2022A25）；深圳市基礎(chǔ)研究面上項目（JCYJ20220531102208019）；深圳大學(xué)2023年度聚徒項目（聚徒+學(xué)術(shù)）