目的 基于斑馬魚模型探究水溶性姜黃素制劑對血栓形成和炎癥消退的作用。方法 隨機(jī)挑選受精后3 d的斑馬魚分為對照組、模型組、陽性藥對照組、水溶性姜黃素制劑（姜黃素質(zhì)量分?jǐn)?shù)為10%，125、250、500、1 000、2 000 μg·mL^-1）組、普通姜黃素（姜黃素質(zhì)量分?jǐn)?shù)為95%，125、250、500、1 000、2 000 μg·mL^-1）組，每組30尾。除對照組外，其余組使用花生四烯酸、脂多糖、五水合硫酸銅分別誘導(dǎo)建立血栓、細(xì)菌性炎癥和神經(jīng)性炎癥模型。通過觀察分析斑馬魚紅細(xì)胞染色強(qiáng)度和炎癥部位中性粒細(xì)胞個數(shù)來評價水溶性姜黃素制劑抑制血栓形成和抗炎作用。結(jié)果 與對照組比較，模型組斑馬魚心臟紅細(xì)胞染色強(qiáng)度明顯減少（P<0.001）、炎癥部位中性粒細(xì)胞個數(shù)明顯增多（P<0.001）；與模型組比較，水溶性姜黃素制劑≥250 μg·mL^-1質(zhì)量濃度時能顯著抑制血栓形成、消退炎癥，主要表現(xiàn)為斑馬魚心臟紅細(xì)胞染色強(qiáng)度顯著升高（P<0.001）、炎癥部位中性粒細(xì)胞個數(shù)明顯減少（P<0.05、0.01、0.001）；姜黃素在≥1 000 μg·mL^-1時能顯著抑制血栓形成（P<0.01、0.001），≥500 μg·mL^-1時能顯著消退炎癥（P<0.05、0.01、0.001）。姜黃素為水溶性姜黃素制劑給藥濃度2～8倍的條件下，才能達(dá)到相同的抑制血栓和炎癥形成的效果。結(jié)論 與普通姜黃素組比較，在相同給藥濃度下，水溶性姜黃素制劑具有更好的抑制血栓形成和抗炎作用。

Objective To explore the effect of water-soluble curcumin on thrombosis and inflammation regression based on zebrafish model. Method Zebrafish three days after fertilization were randomly selected to be divided into control group, model control group, positive drug control group, water-soluble curcumin with different concentrations experimental (curcumin mass fraction 10%, 125, 250, 500, 1 000 and 2 000 μg·mL^-1) groups and ordinary curcumin with different concentrations experimental (turmeric mass fraction 95%, 125, 250, 500, 1 000 and 2 000 μg·mL^-1) groups. Each group has 30 zebrafish. Except for the normal control group, the other experimental groups used Arachidonic acid, Lipopolysaccharide and CuSO₄·5H₂O to induce thrombosis, bacterial inflammation and neurological inflammation, respectively. By observing and analyzing the staining intensity of red blood cells and the number of neutrophils in the inflammation sites of zebrafish, the antithrombotic and anti-inflammatory effects of water-soluble curcumin were evaluated. Results Compared with the control group, the erythrocyte staining intensity of zebrafish heart in model control group was significantly decreased (P< 0.001), and the number of neutrophils in the inflammation site was significantly increased (P< 0.001). Compared with model group, water-soluble curcumin at 250 μg·mL^-1 could significantly inhibit thrombosis and subside inflammation, which were mainly represented by increased erythrocyte staining intensity of zebrafish heart (P< 0.001) and decreased neutrophil number at the inflammation site (P< 0.05, 0.01, 0.001). When curcumin at ≥ 1 000 μg·mL^-1, it can significantly inhibit thrombosis (P< 0.01, 0.001), when ≥ 500 μg·mL^-1, it can significantly reduce inflammation (P< 0.05, 0.01, 0.001). Curcumin can achieve the same inhibitory effect on thrombosis and inflammation only when administered at a concentration of 2-8 times that of water-soluble curcumin preparations. Conclusion Compared with the experimental groups of ordinary curcumin, the water-soluble curcumin showed better antithrombotic and anti-inflammatory effects at the same concentration.

漯河市2019年度重大科技創(chuàng)新專項(xiàng)（201901004）