目的 建立黃花倒水蓮醇Polygala fallax Hemsl.提取物中3種成分的含量測定方法；探討黃花倒水蓮醇提取物抗炎、調(diào)血脂作用。方法 采用HPLC法測定黃花倒水蓮醇提取物中3種成分——細(xì)葉遠(yuǎn)志皂苷、獐牙菜苦苷、蘆丁的含量；建立二甲苯致小鼠耳腫脹、冰醋酸致小鼠腹腔毛細(xì)血管通透性增加、角叉菜膠致小鼠足跖腫脹炎癥模型，評價(jià)黃花倒水蓮醇提取物高、中、低劑量（40、20、10 g·kg^-1）改善急、慢性炎癥的藥理作用；建立二甲苯致摘除雙側(cè)腎上腺小鼠耳腫脹、角叉菜膠致摘除雙側(cè)腎上腺小鼠足跖腫脹模型，判斷其抗炎作用是否依賴下丘腦-垂體-腎上腺軸（HPAA）系統(tǒng)，試劑盒法測定足跖炎癥組織丙二醛（MDA）、一氧化氮（NO）水平和超氧化物歧化酶（SOD）活性；采用高脂飼料喂食SD大鼠建立高脂大鼠模型，考察黃花倒水蓮醇提取物高、中、低劑量（40、20、10 g·kg^-1）對高脂大鼠血清總膽固醇（TC）、三酰甘油（TG）、低密度脂蛋白膽固醇（LDL-C）、高密度脂蛋白膽固醇（HDL-C）水平的影響。結(jié)果 HPLC法方法學(xué)考察各項(xiàng)指標(biāo)符合規(guī)定，細(xì)葉遠(yuǎn)志皂苷、獐牙菜苦苷、蘆丁的平均加樣回收率均在97.13%～101.82%，RSD值均小于3.0%；黃花倒水蓮醇提取物中細(xì)葉遠(yuǎn)志皂苷、獐牙菜苦苷、蘆丁的質(zhì)量分?jǐn)?shù)分別為121.71、84.02、52.53 μg·g-1。與模型組比較，黃花倒水蓮醇提取物高、中劑量對小鼠耳腫脹有明顯的抑制作用（P＜0.05、0.01） ，高、中、低劑量對小鼠腹腔毛細(xì)血管通透增加均有顯著抑制作用（P＜0.05、0.01） ，高、中、低劑量對角叉菜膠造成的小鼠足跖腫脹都有明顯的抑制作用（P＜0.05、0.01）。與模型組比較，在摘除雙側(cè)腎上腺小鼠中，黃花倒水蓮醇提取物高、中劑量能顯著抑制二甲苯致該模型小鼠耳腫脹（P＜0.05、0.01） ，顯著抑制角叉菜膠致該模型小鼠足跖腫脹（P＜0.05、0.01） ；黃花倒水蓮醇提取物高、中、低劑量均能顯著降低足跖中MDA、NO水平（P＜0.05、0.01） ，均能顯著提高足跖中SOD活性（P＜0.05、0.01）。ig高脂飼料28 d后，與模型組比較，黃花倒水蓮醇提取物高、中、低劑量能顯著降低大鼠血清的TC、TG和LDL-C水平（P＜0.05）。結(jié)論 建立的HPLC含量測定方法專屬性強(qiáng)、重復(fù)性好、準(zhǔn)確度高，可用于黃花倒水蓮醇提取物中細(xì)葉遠(yuǎn)志皂苷、獐牙菜苦苷、蘆丁的含量測定；黃花倒水蓮醇提取物具有抗炎活性，其抗炎作用的發(fā)揮不依賴HPAA系統(tǒng)，與減少M(fèi)DA、NO水平，提高SOD活性有關(guān)；對高脂大鼠發(fā)揮明顯的調(diào)血脂作用。

Objective To establish a method for the determination of three components in Polygala fallax alcohol extract (PFAE) to investigate the anti-inflammatory and hypolipidemic effects of PFAE. Methods The contents of three components (tenuifolin, swertiamarin and rutin) in PFAE were determined by HPLC. The inflammatory models such as xylene induced ear swelling in mice, acetic acid induced increased permeability of abdominal capillaries in mice, and carrageenan induced paw swelling inflammation in mice models were established to evaluate the pharmacological effects of high, medium and low doses (40, 20, and 10 g·kg^-1) PFAE on acute and chronic inflammation. The ear swelling model of mice with bilateral adrenalectomy induced by xylene and the paw swelling model of mice with bilateral adrenalectomy induced by carrageenan were established to determine whether its antiinflammatory effect depends on the hypothalamic-pituitary-adrenal axis (HPAA) system. The contents of malondialdehyde (MDA) and nitric oxide (NO) and the activity of superoxide dismutase (SOD) in the inflammatory tissue of the paw were determined by kits. A high-fat rat model was established by feeding SD rats with high-fat feed, and the effects of high, medium, and low doses (40, 20, and 10 g·kg^-1) of PFAE on serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and highdensity lipoprotein cholesterol (HDL-C) levels in hyperlipidemic rats were investigated. Results The indexes of methodological investigation were in accordance with the regulations. The average recovery rates of tenuifolin, swertiamarin and rutin were 97.13%—101.82%, and the RSD values were less than 3.0%. The mass fractions of tenuifolin, swertiamarin and rutin in the extract were 121.71, 84.02 and 52.53 μg·g-1, respectively. Compared with model group, except for the low dose group was not statistically significant, the high and middle dose groups of PFAE had significant inhibition on ear swelling in mice (P＜0.05, 0.01). The high, medium and low dose groups of PFAE had obvious inhibition on the permeability of abdominal capillary in mice (P＜0.05, 0.01), and the swelling of foot caused by carrageenan had significant inhibition (P＜0.05, 0.01). Compared with model group, in the mouse model of bilateral adrenalectomy, the high and medium dose groups of PFAE could significantly inhibit the ear swelling of the model mice induced by xylene (P＜0.05, 0.01), and can significantly inhibit carrageenan-induced paw swelling in the model mice (P＜0.05, 0.01), the high, medium and low dose groups of PFAE could significantly reduce the levels of MDA and NO in the toes, and significantly increase the content of SOD in the toes (P＜0.05, 0.01). After 28 days of high-fat diet, compared with the model group, high, medium, and low doses of PFAE significantly reduced the levels of TC, TG, and LDL-C in rat serum (P＜0.05). Conclusion The content determination method established in this study has strong specificity, good repeatability and high accuracy, which can be used for the content determination of tenuifolin, swertiamarin and rutin in PFAE. PFAE has anti-inflammatory activity, and its anti-inflammatory effect may not depend on the hypothalamic pituitary adrenal axis (HPAA) system. At the same time, the anti-inflammatory mechanism of PFAE is related to reducing MDA, NO and increasing SOD activity. PFAE has obvious hypolipidemic effect on experimental hyperlipidemia.

R284.1；R285.5

國家自然科學(xué)基金資助項(xiàng)目（81360524）；2020年度廣西高校中青年教師科研基礎(chǔ)能力提升項(xiàng)目（2020KY13034）；廣西壯族自治區(qū)中醫(yī)藥管理局科研課題（GZZC2019147）