目的 建立多指標(biāo)成分定量聯(lián)合化學(xué)計(jì)量學(xué)、加權(quán) TOPSIS 與灰色關(guān)聯(lián)度融合模型評(píng)價(jià)不同產(chǎn)地纈草 Valeriana officinalis L. 質(zhì)量差異的方法。方法 采用高效液相色譜法，以 Phenomenex Gemini C₁₈色譜柱為固定相，乙腈-0.05% 磷酸為流動(dòng)相，梯度洗脫，對(duì) 16 批纈草中橙皮苷、香葉木素、纈草三酯、乙酰纈草三酯、氯化纈草素、羥基纈草烯酸、乙酰氧基纈草酸、纈草烯酸、β-谷甾醇和熊果酸含量進(jìn)行同步檢測(cè)。結(jié)合化學(xué)計(jì)量學(xué)、加權(quán) TOPSIS 與灰色關(guān)聯(lián)度分析（GRA）融合模型進(jìn)行質(zhì)量差異的全面評(píng)價(jià)。結(jié)果 HPLC 方法學(xué)驗(yàn)證符合中國(guó)藥典要求，10 種成分在各自范圍內(nèi)線性關(guān)系良好（R²＞0.999），平均加樣回收率（n＝9）在 96.83%～100.08%（RSD＜2.0%）?；瘜W(xué)計(jì)量學(xué)結(jié)果顯示 16 批纈草明顯分為 3 類，乙酰氧基纈草酸、橙皮苷、纈草三酯和羥基纈草烯酸為貢獻(xiàn)度較大的差異性標(biāo)志物。加權(quán) TOPSIS 與灰色關(guān)聯(lián)度融合結(jié)果顯示16 批樣品的相對(duì)貼近度為 0.279 2～0.666 5，湖北和貴州產(chǎn)地的纈草整體質(zhì)量較好（相對(duì)貼近度為 0.5790～0.6665）。結(jié)論 以橙皮苷等 10 種成分含量為評(píng)價(jià)指標(biāo)，采用化學(xué)計(jì)量學(xué)、加權(quán) TOPSIS 與灰色關(guān)聯(lián)度融合模型可用于評(píng)價(jià)纈草的質(zhì)量。

Objective To establish a method to evaluate the quality difference of Valeriana officinalis from different producing areas by multi-index component quantitative combined with chemometrics, weighted TOPSIS and grey correlation degree fusion model. Methods Using HPLC to detection the contents of hesperidin, diosmetin, valtrate, acevaltrate, valechlorine, hydroxyvalerenic acid, acetoxyvalerenic acid, valerenic acid, β-sitosterol and ursolic acid in 16 batches of V. officinalis samples with Phenomenex Gemini C₁₈ column, and mobile phase was consisted of acetonitrile-0.05% phosphoric acid solution, gradient elution. The quality difference was comprehensively assessed by chemometrics, weighted TOPSIS and GRA fusion models. Results The validation of HPLC methodology met the requirements of Chinese Pharmacopoeia. The 10 components showed good linearity in their ranges (R² > 0.999), and the average recovery (n＝ 9) were 96.83%—100.08% (RSD < 2.0%). The results of chemometrics analysis showed that 16 batches of Valeriana officinalis were divided into three categories, and acetoxyvalerenic acid, hesperidin, valtrate and hydroxyvalerenic acid were the main markers of the quality differences. The results of weighted TOPSIS and grey correlation degree fusion technology showed that the relative closeness of 16 batches of samples was 0.279 2—0.666 5, and the overall quality of Valerian from Hubei and Guizhou provinces was good (relative closeness was 0.579 0—0.666 5). Conclusion With the content of 10 components such as hesperidin as the evaluation index, the fusion model of chemometrics, weighted TOPSIS and grey relational analysis fusion model can be used to evaluate the quality of Valeriana Officinalis.

R969.1

山東省藥品化妝品監(jiān)測(cè)哨點(diǎn)課題項(xiàng)目（2022SDADRKY09）