目的 通過氣相色譜-質(zhì)譜（GC-MS）技術(shù)對(duì)經(jīng)典名方當(dāng)歸補(bǔ)血湯在飲片、基準(zhǔn)樣品、提取液、濃縮液、浸膏粉及顆粒中的揮發(fā)性成分進(jìn)行檢測(cè)，建立多指標(biāo)成分含量測(cè)定方法，探究其量值傳遞規(guī)律。方法 采用GC-MS技術(shù)對(duì)當(dāng)歸補(bǔ)血湯基準(zhǔn)樣品中揮發(fā)性成分進(jìn)行指認(rèn)，判定其成分歸屬，借助NIST 20s.lib數(shù)據(jù)庫及對(duì)照品比對(duì)篩選出共有成分，建立共有成分的GC-MS含量測(cè)定方法，探究其在飲片-基準(zhǔn)樣品、飲片-提取液、提取液-濃縮液、濃縮液-浸膏粉、浸膏粉-顆粒中的量值傳遞規(guī)律。結(jié)果 當(dāng)歸補(bǔ)血湯基準(zhǔn)樣品中共檢出10個(gè)響應(yīng)較好的成分，其中2個(gè)來自黃芪，8個(gè)來自酒當(dāng)歸，篩選出苯甲酸、2-甲氧基-4-乙烯基苯酚、藁本內(nèi)酯、洋川芎內(nèi)酯H 4個(gè)共有成分。含量測(cè)定結(jié)果表明此4個(gè)成分從飲片到顆粒得到有效傳遞，且苯甲酸、2-甲氧基-4-乙烯基苯酚和洋川芎內(nèi)酯H在顆粒的轉(zhuǎn)移率與基準(zhǔn)樣品較接近，而藁本內(nèi)酯在濃縮和噴干工藝中損失較多，導(dǎo)致轉(zhuǎn)移率相差較大。結(jié)論 從飲片到顆粒的傳遞過程中，不同揮發(fā)性成分的傳遞規(guī)律不一致，有必要優(yōu)選合適生產(chǎn)工藝保留揮發(fā)性成分，并選擇合理評(píng)價(jià)指標(biāo)進(jìn)行質(zhì)量控制。

Objective The volatile components of the classic formula Danggui Buxue Decoction were detected by GC-MS in tablets, baseline samples, extract powder, concentrates, extracts and granules, to establish a multi-indicator method for the determination of the content of the components and to investigate the transmission law of the quantitative value. Method The volatile components in the baseline sample of Danggui Buxue Decoction were identified by GC-MS, and the attribution of the components was determined. With the help of NIST 20s.lib database and standard comparison, the common components were screened out, and the method for the determination of the content of the common components by GC-MS was established, and the quantitative value transfer patterns were investigated in the tablets-baseline samples, tablets-extracts, extracts-concentrates, concentrates-extract powder, extract powdergranules. Results In the baseline sample of Danggui Buxue Decoction, a total of 10 components were detected, among which two components were from Astragalus membranaceus (Fisch.) Bunge and eight components were from Angelica sinensis, and four common components, including benzoic acid, 2-methoxy-4-vinylphenol, ligustilide, and senkynolide H, were screened out. The results showed that these four components were effectively transferred from tablets to granules, and the transfer rates of benzoic acid, 2-methoxy-4-vinylphenol and senkynolide H to granules were close to those of the baseline samples, whereas ligustilide was more lost in the concentration and spray-drying processes, resulting in a larger difference in the transfer rates. Conclusion The transfer pattern of different volatile components from slices to granules was inconsistent, and it is necessary to prefer suitable production processes to retain the volatile components and select reasonable evaluation indexes for quality control.

R917

國(guó)家自然科學(xué)基金項(xiàng)目（82174209）