目的 評價益胃湯加味對乙醇誘導(dǎo)胃潰瘍模型大鼠的保護(hù)作用。方法 SD大鼠隨機(jī)分為對照組、模型組及益胃湯加味低、中、高劑量（0.8、1.2、1.7 g·kg^-1）組，每組8只，各組均按劑量預(yù)給藥30 d后，全部動物嚴(yán)格禁食24 h（不禁水）。除對照組外，其余大鼠每只均ig給予無水乙醇1.0 mL，1 h后犧牲動物取材。暴露完整胃，展開胃黏膜面拍照，對損傷進(jìn)行評分；取部分胃組織進(jìn)行蘇木精-伊紅（HE）染色；另取部分胃組織測定髓過氧化物酶（MPO）、總超氧化物歧化酶（SOD）、前列腺素E₂（PGE₂）、胃蛋白酶原Ⅰ（PGⅠ）、胃蛋白酶原Ⅱ（PGⅡ）水平；取血清檢測其中血管內(nèi)皮生長因子（VEGF）、白細(xì)胞介素-6（IL-6）、白細(xì)胞介素-1β（IL-1β）、腫瘤壞死因子-α（TNF-α）水平。此外，體外培養(yǎng)人胃黏膜上皮細(xì)胞（GES-1）及人臍靜脈內(nèi)皮細(xì)胞（HUVEC），通過MTT與劃痕實驗，評價益胃湯加味對增殖及遷移的作用。結(jié)果 益胃湯加味可顯著減輕胃黏膜損傷和降低胃黏膜病理評分（P＜0.001）；顯著降低胃MPO活力并提高SOD活力（P＜0.05、0.001）；顯著提高胃PGE₂、PG Ⅰ、PG Ⅱ水平（P＜0.05、0.001）；顯著降低血清TNF-α、IL-1β、IL-6水平（P＜0.01、0.001）；增加血清VEGF水平（P＜0.05），促進(jìn)GES-1和HUVEC細(xì)胞增殖與遷移（P＜0.01、0.001）。結(jié)論 益胃湯加味可通過抑制炎癥因子分泌，緩解機(jī)體氧化應(yīng)激，保護(hù)胃黏膜以及促進(jìn)細(xì)胞增殖遷移等多種途徑發(fā)揮對胃潰瘍模型大鼠的保護(hù)作用。

Objective To evaluate the protective effect of Modified Yiwei Decoction on ethanol-induced gastric ulcer model rats. Methods SD rats were randomly divided into control group, model group and Modified Yiwei Decoction low-, medium-and highdose group (0.8, 1.2 and 1.7 g·kg^-1), eight rats in each group. The drugs were given by ig administration before the model was established, once a day for 30 d, all the rats were strictly abstinence for 24 h (water freedom). Excluding rats in the control group, the rest of the rats were given 1 mL of absolute ethanol by ig administration to induce gastric ulcers. One hour later, all rats were sacrificed immediately. The gastric mucosal surface was unfolded to take pictures and the score of gastric damage was measured. HE staining of stomach sections were carried out to observe pathological changes. Myeloperoxidase (MPO), total superoxide dismutase (SOD), prostaglandin E₂ (PGE₂), pepsinogen Ⅰ (PGⅠ), pepsinogen Ⅱ (PGⅡ), vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were detected. In addition, human gastric mucosal epithelial cells (GES-1) and human umbilical vein endothelial cells (HUVEC) were cultured in vitro, and the effects of Modified Yiwei Decoction on proliferation and migration were evaluated by MTT and scratch tests. Results Modified Yiwei Decoction can significantly reduce the damage of gastric mucosa and the pathological score of gastric mucosa (P < 0.001). Gastric MPO activity was significantly decreased and serum SOD activity was increased (P < 0.05, 0.001). The contents of PGE₂, PG Ⅰ and PG Ⅱ in stomach were significantly increased (P < 0.05, 0.001). The contents of TNF-α, IL-1β and IL-6 in serum were significantly decreased (P < 0.01, 0.001). And increased serum VEGF level (P < 0.05) Promote proliferation and migration of GES-1 and HUVEC cells (P < 0.01, 0.001). Conclusion Modified Yiwei Decoction can inhibit the secretion of inflammatory factors, relieve oxidative stress, protect gastric mucosa, promote cell proliferation and migration, and play a protective role in gastric ulcer model rats.

R285.5

中央高校基本科研業(yè)務(wù)費專項資金資助項目（2632023TD06）