目的 探討丁香花蕾乙醇提取物（AEC）在 39 ℃條件下對(duì)口腔鱗狀細(xì)胞癌增殖、遷移、凋亡的影響。方法 設(shè)置空白對(duì)照組、溶劑對(duì)照組和0.05%、0.10%、0.20% AEC組，分別于39 ℃條件下作用于口腔鱗狀細(xì)胞癌細(xì)胞培養(yǎng)72 h。采用CCK-8和克隆形成法檢測口腔鱗狀細(xì)胞癌的增殖能力；劃痕實(shí)驗(yàn)檢測細(xì)胞的遷移能力；流式細(xì)胞術(shù)和 Hoechest 33342染色檢測細(xì)胞的凋亡。結(jié)果 與對(duì)照組和模型組比較，0.05%、0.10%、0.20%組口腔鱗狀細(xì)胞增殖活性逐漸降低，細(xì)胞克隆形成隨著濃度增加集落形成降低，細(xì)胞遷移能力和侵襲能力逐漸減弱；細(xì)胞凋亡率逐漸增加，均與 AEC 呈濃度相關(guān)性。在39 ℃條件下，口腔鱗狀細(xì)胞癌增殖、遷移和侵襲的抑制作用更加明顯，細(xì)胞凋亡更加顯著。結(jié)論 AEC對(duì)口腔鱗狀細(xì)胞癌的增殖、遷移具有抑制作用，且能促進(jìn)口腔鱗狀細(xì)胞癌凋亡，在39 ℃條件下，具有協(xié)同作用，對(duì)口腔鱗狀細(xì)胞癌增殖、遷移、凋亡的作用更明顯。

Objective To investigate the effects of alcohol extract of clove (AEC) combined with 39 ℃ on the proliferation, migration and apoptosis of oral squamous cell carcinoma (OSCC). Methods Oral squamous cells (OSC) were cultured with 0, 0.05%, 0.10%, or 0.20% AEC at 39 ℃ for 72 h. CCK-8 and colony formation assays were used to detect the proliferation of OSCC. The migration ability of cells was detected by scratch test. The apoptosis of the cells was detected by flow cytometry and Hoechest 33342 staining. Results Compared with the control and model group, the 0.05%, 0.10% and 0.20% groups showed a gradual decrease in the proliferation activity of OSC. With the increase of the concentration of 0.05%, 0.10%, and 0.20% groups, the colony formation decreased, and the cell migration and invasion ability gradually decreased. The apoptosis rate of OSC increased gradually, both in a concentration dependent manner with AEC. Combined with 39 ℃ treatment, the inhibition of proliferation, migration and invasion of OSC was more obvious, and cell apoptosis was more significant. Conclusion AEC can inhibit the proliferation and migration of OSCC, and promote the apoptosis of OSCC. When used in combination with 39 ℃, it has a synergistic effect, so as to achieve the therapeutic effect of OSCC.

R285.5

國家自然科學(xué)基金資助項(xiàng)目（82060489）；貴州省科技技術(shù)廳資助項(xiàng)目（黔科合基礎(chǔ)-ZK［2024］一般 404）