目的 建立同時(shí)測(cè)定白蘞中沒食子酸、原兒茶酸、槲皮素、兒茶素、白蘞素、白藜蘆醇、大黃素、大黃素甲醚、齊墩果酸、羽扇豆醇、豆甾醇、胡蘿卜苷、β-谷甾醇含量的方法，結(jié)合化學(xué)計(jì)量學(xué)、加權(quán)TOPSIS模型，評(píng)價(jià)不同產(chǎn)地白蘞的質(zhì)量。方法 采用HPLC法，應(yīng)用Prism RP C₁₈色譜柱，乙腈-0.2%磷酸為流動(dòng)相，梯度洗脫，采用280、254和210 nm三波長測(cè)定18批白蘞中上述13個(gè)成分含量，同時(shí)檢測(cè)醇溶性浸出物（ ESE）、總灰分和酸不溶性灰分。結(jié)合化學(xué)計(jì)量學(xué)、加權(quán)TOPSIS法綜合評(píng)價(jià)白蘞的整體質(zhì)量。結(jié)果 13個(gè)成分在65 min內(nèi)完全分離，在一定質(zhì)量濃度范圍內(nèi)的線性關(guān)系良好，平均加標(biāo)回收率為96.94%～100.10%，RSD均＜2.0%； 18批白蘞中13個(gè)成分含量分別為（ 0.509±0.110）、（ 0.672±0.086）、（ 1.498±0.255）、（ 3.597±0.601）、（ 0.246±0.080）、（ 0.248±0.051）、（ 0.141±0.029）、（ 0.183±0.044）、（ 0.113±0.040）、（0.387±0.085）、（0.052±0.025）、（0.069±0.014）、（0.343±0.076） mg·g-1，ESE、總灰分和酸不溶性灰分含量分別為（20.0±2.0）%、（ 9.0±2.4）%和（ 2.2±0.8）%，16個(gè)指標(biāo)含量差異均較大。化學(xué)計(jì)量學(xué)分析結(jié)果顯示18批白蘞聚為3類，以VIP值＞1為閾值，篩選出兒茶素、槲皮素、羽扇豆醇、原兒茶酸和β-谷甾醇為區(qū)分18個(gè)不同產(chǎn)地白蘞的差異性標(biāo)志物。因子分析（ FA）和加權(quán)TOPSIS法均顯示安徽、河南、湖北產(chǎn)地的白蘞質(zhì)量較優(yōu)。結(jié)論 基于HPLC法同時(shí)測(cè)定白蘞中13個(gè)成分含量方法，該方法準(zhǔn)確可靠，化學(xué)計(jì)量學(xué)與加權(quán)TOPSIS法科學(xué)直觀，可用于評(píng)價(jià)白蘞的整體質(zhì)量。

Objective To establish a method for simultaneous determination of gallic acid, protocatechuic acid, quercetin, catechin, trichosanthin, resveratrol, emodin, physcion, oleanolic acid, lupeol, stigmasterol, daucosterol and β-sitosterol in Ampelopsis radix. and to evaluate the quality of A. radix from different origins by chemometrics and weighted TOPSIS model. Methods HPLC was used with a Prism RP C₁₈ column and a mobile phase of acetonitrile-0.2% phosphoric acid with gradient elution. The contents of the 13 components were determined at 280, 254 and 210 nm. The ethanol-soluble extractives (ESE), total ash and acid-insoluble ash were also determined. The overall quality of A. radix was comprehensively evaluated by chemometrics and weighted TOPSIS method. Results The 13 components were completely separated within 65 min. Good linear relationships were obtained within the tested concentration ranges. The average recovery rates were 96.94% to 100.10% with RSD all less than 2.0%. The contents of the 13 components in 18 batches of A. radix were (0.509 ±0.110), (0.672 ±0.086), (1.498 ±0.255), (3.597 ±0.601), (0.246 ±0.080), (0.248 ±0.051), (0.141 ±0.029), (0.183 ±0.044), (0.113 ±0.040), (0.387 ±0.085), (0.052 ±0.025), (0.069 ±0.014), (0.343 ±0.076) mg·g-1, respectively. The contents of ethanol-soluble extractives (ESE), total ash and acid-insoluble ash were (20.0 ±2.0)%, (9.0 ±2.4)% and (2.2 ±0.8)%, respectively. The contents of the 16 indicators varied greatly. Chemometrics analysis showed that the 18 batches of A. radix were clustered into 3 categories. With VIP value > 1 as the threshold, catechin, quercetin, lupeol, protocatechuic acid and β- sitosterol were selected as the differential markers for distinguishing A. radix from different origins. Both factor analysis and weighted TOPSIS method indicated that the quality of A. radix from Anhui, Henan and Hubei was relatively superior. Conclusion The HPLC method for simultaneous determination of 13 components in A. radix is accurate and reliable. Chemometrics and weighted TOPSIS method are scientific and intuitive, and can be used to evaluate the overall quality of A. radix.

R286.2

河南省醫(yī)學(xué)教育研究項(xiàng)目（WJLX2024254）；河南省醫(yī)學(xué)教育研究項(xiàng)目（WJLX2020326）；湖北省高等學(xué)校優(yōu)秀中青年科技創(chuàng)新團(tuán)隊(duì)計(jì)劃項(xiàng)目（T2023039）