目的 基于自然殺傷（NK）細(xì)胞相關(guān)文獻(xiàn)分析探究NK細(xì)胞的研究現(xiàn)狀、熱點(diǎn)和發(fā)展趨勢。方法 以中國學(xué)術(shù)期刊全文數(shù)據(jù)庫（CNKI）、維普生物醫(yī)學(xué)數(shù)據(jù)庫（VIP）、萬方數(shù)據(jù)庫（Wanfang Data）、PubMed和Web of Science等中英文數(shù)據(jù)庫收錄的NK細(xì)胞相關(guān)的文獻(xiàn)為研究對象，采用CiteSpace6.2.R2軟件進(jìn)行分析。結(jié)果 共納入11 653篇與NK細(xì)胞相關(guān)的中英文文獻(xiàn)，文獻(xiàn)年發(fā)表量總體逐漸上升，主要作者有周忠海、劉軍權(quán)、Wang Y等，主要研究機(jī)構(gòu)有中國科學(xué)技術(shù)大學(xué)免疫學(xué)研究所、上海中醫(yī)藥大學(xué)和Tabriz University of Medical Sciences等，高頻關(guān)鍵詞主要有免疫功能、免疫治療、腫瘤、免疫調(diào)節(jié)、腫瘤微環(huán)境等。結(jié)論 當(dāng)前NK細(xì)胞研究熱點(diǎn)主要集中于其抗腫瘤應(yīng)用，利用NK細(xì)胞接合器殺傷腫瘤顯示出更好的安全性與療效，為NK細(xì)胞免疫療法提供重要意義；在NK細(xì)胞培養(yǎng)方法方面的關(guān)注較少，日后可挖掘能夠有效激活NK細(xì)胞的中藥活性成分，增加聯(lián)合中藥培養(yǎng)NK細(xì)胞的方法及機(jī)制研究，為培養(yǎng)NK細(xì)胞提供新思路與新方向，同時促進(jìn)中藥理論與免疫細(xì)胞的融合發(fā)展。

Objective To explore the current research status, hotspots and development trends of natural killer cells (NK cells) based on the analysis of NK cells related literature. Methods Literature related to NK cells included in Chinese and English databases, such as CNKI, VIP Database, Wanfang Database, PubMed and Web of Science, were used as the study object and analyzed by CiteSpace 6.2.R2 software. Results A total of 11 653 NK cell-related papers in English and Chinese were included, with an overall gradual increase in the annual publication volume of the literature. The main authors include Zhou Zhonghai, Liu Junquan, Wang Y, and others, and the main research institutes were the Institute of Immunology, University of Science and Technology of China, the Shanghai University of Traditional Chinese Medicine, and the University of Medical Sciences in Tabriz, etc. The main high-frequency keywords are immune function, immunotherapy, tumor, immune regulation, tumor microenvironment and so on. Conclusion The current hotspots of NK cells research mainly focuses on its anti-tumor application, and the use of NK cells splicer to kill tumors shows better safety and efficacy, which provides an important significance for NK cells immunotherapy; less attention has been paid to NK cells culture methods, and in the future, we can explore the active ingredients of traditional Chinese medicine that can effectively activate NK cells, and increase the research on the methods and mechanisms of NK cells culture with traditional Chinese medicine, which will provide new ideas and new directions for the cultivation of NK cells, and promote the integration and development of traditional Chinese medicine theory and immune cells at the same time.

R979.1

教育部產(chǎn)學(xué)合作協(xié)同育人項目（22097040193735）