目的 初步研究附子、紅參不同比例配伍對(duì)大鼠腸道菌群的影響。方法 紅參、附子分別采用8倍體積的70%乙醇加熱回流提取2次，每次1 h，制備醇提物。將15只SD大鼠隨機(jī)分成A、B、C 3組，每組5只，A組為附子醇提物單獨(dú)給藥組，給藥量為0.415 g/kg，B、C組分別為附子、紅參配伍比例1∶1（紅參醇提物1.17 g/kg）和1∶2（紅參醇提物2.34 g/kg）給藥組，每天ig給藥1次，連續(xù)給藥7 d。給藥結(jié)束后，于第8天收集大鼠糞便，提取糞便中細(xì)菌的DNA，對(duì)糞便菌群的16Sr-RNA的V4區(qū)進(jìn)行擴(kuò)增，利用Miseq高通量測(cè)序平臺(tái)進(jìn)行基因序列的測(cè)定，利用Uparse software對(duì)序列進(jìn)行分析，將相似性在97%以上的序列進(jìn)行歸并，生成分類操作單元（OTU）；利用QⅡME軟件計(jì)算樣品的菌群豐度指數(shù)（Ace、Chao1）、菌群多樣性指數(shù)（Simpson、Shannon）。結(jié)果 A組和B組的OTU數(shù)量較C組顯著增多（P<0.05）；A組和B組Ace、Chao1指數(shù)為1 393、1 368和1 085、1 057，C組Ace和Chao1指數(shù)為889和884，A組和B組較C組顯著增多（P<0.05、0.01）；厚壁菌門、擬桿菌們和變形菌門均為3組樣品中的優(yōu)勢(shì)菌門；在屬的水平上，C組中乳酸桿菌屬和擬桿菌屬含量較A組和B組增加。結(jié)論 紅參與附子不同比例配伍可對(duì)大鼠腸道菌群產(chǎn)生顯著的影響，隨紅參比例的增加，可能在一定程度上改善腸道的微生態(tài)環(huán)境。

Objective To analyze the effect of AconitiLateralis Radix Praeparata (ALRP) and Red Ginseng in different compounding ratios on the intestinal microbiota of rats by using Miseq high-throughput sequencing technology.Methods Red Ginseng and ALRP were extracted by refluxing with 70% ethanol of eight times volume for two times, 1 h each time, to prepare ethanol extracts. Fifteen SD rats were randomly divided into A, B and C group, which were treated with ALRP ethanol extracts alone, ALRP and Red Ginseng in compounding ratio of 1:1 (Red Ginseng ethanol extracts 1.17 g/kg) and 1:2 (Red Ginseng ethanol extracts 2.34 g/kg) respectively, ig administrated once daily for 7 d. The fecal samples were collected on the 8th to extract bacterial DNA. The fecal bacteria composition was investigated by Miseq high-throughput sequencing of the V4 region of the 16Sr-RNA gene. Uparse Software was used to analyze the sequences, and the sequences with 97% similarity were merged to generate OTU, and QⅡME Software was used to calculate the bacterial abundance index (Ace and Chao1) and bacterial diversity index (Simpson and Shannon). Results The number of OTUs in group A and group B was significantly higher than that in group C (P<0.05); the Ace and C hao1 indices in group A and group B were 1 393 and 1 368, 1 085 and 1 057, and the Ace and Chao1 indices in group C were 889 and 884, while the A and B groups were significantly higher than that in group C (P<0.05 and 0.01); Fimicutes, Bacteroides and Proteobacteria were the dominant phylum in the three groups; at the genus level, Lactobacillus and Bacteroides in group C were the dominant phylum. Conclusion ALRP and Red Ginseng in different compounding ratio have significant effect on gut microbiota. With the increase of the proportion of Red Ginseng, the intestinal microecological environment may be improved to a certain extent.

國(guó)家自然科學(xué)基金資助項(xiàng)目（81503457，81673824）