目的 建立不同產(chǎn)地土貝母藥材中皂苷類成分的特征圖譜，并測定其中3種主要皂苷的含量，為該藥材質(zhì)量控制提供參考依據(jù)。方法 采用Thermo Hypersil GOLD aQ C₁₈色譜柱（250 mm×4.6 mm，5 μm），乙腈-水為流動相進行梯度洗脫，體積流量1.0 mL/min，進樣量10 μL，柱溫30℃，檢測波長214 nm，建立15批土貝母HPLC特征圖譜，并進行相似度評價、主成分分析及聚類分析；采用Diamonsil C₁₈色譜柱（250 mm×4.6 mm，5 μm），以乙腈-水為流動相進行等度洗脫測定藥材中3種皂苷類成分的含量。結(jié)果 建立的15批土貝母特征圖譜共標定6個共有峰，相似度為0.845～0.98；主成分分析及聚類分析均將15批藥材分為兩類，與相似度評價結(jié)果一致；得到影響藥材質(zhì)量的3個皂苷成分（土貝母苷甲、乙、丙）并對其進行了含量測定，結(jié)果 15批藥材都符合藥典含量限度的要求。結(jié)論 本研究所建立的HPLC特征圖譜結(jié)合含量測定以及相似度評價，主成分分析，聚類分析等評價方法，可以全面、準確和有效地用于土貝母藥材的質(zhì)量評價。

Objective To establish the HPLC characteristic spectrum of Bolbostemma paniculatum from different areas, determine the contents of three saponinsa, and provide basis for quality control of B. paniculatum. Methods For characteristic spectrum, chromatographic experiments were performed on an Thermo Hypersil GOLD aQ C₁₈ column (250 mm×4.6 mm, 5 μm) using gradient elution with acetonitrile-aqueous as the mobile phase at a flow rate of 1.0 mL/min, the detection wavelength was 214 nm, the sample size was 10 μL, and the column temperature was set at 30℃. In the end, similarity evaluation, principal component analysis and cluster analysis were carried out. An HPLC method for quantitative analysis was applied with an Diamonsil C₁₈ column (250 mm×4.6 mm, 5 μm) using isocratic elution with acetonitrile-aqueous as the mobile phase at a flow rate of 1.0 mL·min-1, the detection wavelength was 214 nm, the sample size was 10 μL, and the column temperature was set at 30℃.Results There were 6 common peaks identified in the characteristic spectra of 15 batches of B. paniculatum, and the similarity was between 0.845-0.98. All batches of samples can be classified into two groups by using PCA and HCA, which were in accordance with the results of similarity evaluation. The three key components (tubeimoside I, Ⅱ and Ⅲ) affecting the quality of the medicinal materials were identified and their contents were determined.Conclusion The HPLC characteristic spectrum, similarity evaluation, PCA, CA and content determination method can comprehensively, accurately and effectively evaluate the quality of B. paniculatum.

北京中醫(yī)藥大學研究生自主課題項目（2018-JYB-XS）