目的 建立同時(shí)測(cè)定對(duì)乙酰氨基酚、馬來(lái)酸氯苯那敏、綠原酸、牛蒡苷含量的方法，旨在實(shí)現(xiàn)準(zhǔn)確、高效、實(shí)用地控制維C銀翹片的質(zhì)量。方法 采用HPLC波長(zhǎng)切換方法，色譜柱為DIONEX Acclaim C₁₈ （250 mm×4.6 mm，5 μm，Dionex Bonded Silica Products），流動(dòng)相為乙腈-含0.5%三乙胺的0.02 mol/L磷酸二氫鉀（pH2.5）水溶液，梯度洗脫，體積流量為1.0 mL/min，柱溫30℃，檢測(cè)波長(zhǎng)249 nm（0～10 min，檢測(cè)對(duì)乙酰氨基酚）、327 nm（10～20 min，檢測(cè)綠原酸）、264 nm（20～32 min，檢測(cè)馬來(lái)酸氯苯那敏）、280 nm（32～44 min，檢測(cè)牛蒡苷）。結(jié)果 對(duì)乙酰氨基酚、綠原酸、馬來(lái)酸氯苯那敏、牛蒡苷分別在0.040～0.800、0.065～1.301、0.041～0.818、0.093～1.852 μg內(nèi)與峰面積呈良好的線性關(guān)系（r>0.999），平均加樣回收率（n=6）分別為99.7%（RSD=1.4%）、100.4%（RSD=1.2%）、100.5%（RSD=1.3%）、99.4%（RSD=1.5%）。對(duì)乙酰氨基酚、綠原酸、牛蒡苷采用2種方法測(cè)定，經(jīng)t檢驗(yàn)結(jié)果無(wú)顯著性差異；馬來(lái)酸氯苯那敏采用本文建立的方法測(cè)定，含量略高于藥典方法。結(jié)論 本實(shí)驗(yàn)建立的含量測(cè)定方法與《中國(guó)藥典》收載的方法比較，本方法檢測(cè)結(jié)果可靠，且操作簡(jiǎn)單、環(huán)境友好、靈敏度高，符合方法學(xué)驗(yàn)證要求，可用于維C銀翹片的質(zhì)量控制。

Objective To establish a method for Simultaneous determination of four constituents (paracetamol; chlorpheniramine maleate; chlorogenicacid; arctiin), in order to control the quality of Vitamin C Yinqiao Tablets accurately,efficiently and practically. Methods In the HPLC with switching wavelength method, the separation was performed on a DIONEX Acclaim C₁₈ (250 mm×4.6 mm, 5 μm, Dionex Bonded Silica Products) column with the gradient elution of acetonitrile-0.5% triethylamine 0.02 mol/L NaH₂PO₄ (pH2.5) solution in a gradient mode at the flow rate of 1.0 mL/min. The column temperature was set at 30℃. The UV detection wavelength was set at 249 nm for paracetamol in 0-10 min, 327 nm for chlorogenic acid in 10-20 min, 264 nm for chlorpheniramine maleate for 20-32 min, 280 nm for arctiin in 32-44 min. Results The ranges for linear correlation of paracetamol, chlorpheniramine maleate, chlorogenicacid, arctiin were 0.040-0.800, 0.065-1.301, 0.041-0.818, 0.093-1.852 μg. The average recoveries (n=6) of the four components were 99.7% (RSD=1.4%), 100.4% (RSD=1.2%), 100.5% (RSD=1.3%), 99.4% (RSD=1.5%). The t-test results of Paracetamol, Chlorogenicacid and arctiin by both method have no significant difference, while the result of Chlorpheniramine maleate by established method is slightly higher than the corresponding method in Chinese Pharmacopoeia.Conclusion Compared to the corresponding method in Chinese Pharmacopoeia, the established method is reliable and consistent with method validation requirements, which has the characteristic of easy maneuverability,environmental friendliness, high sensitivity, therefore can be used for the quality control of vitamin C Yinqiao tablets.