目的 探討熄風(fēng)靜寧顆粒（XFJNG）對(duì)多發(fā)性抽動(dòng)癥（TS）模型大鼠HPA軸及紋狀體單胺類神經(jīng)遞質(zhì)的影響。方法 采用連續(xù)7 d ip亞氨基二丙腈（IDPN，150 mg/kg）建立TS大鼠模型，造模成功后將大鼠隨機(jī)分為模型組、氟哌啶醇（0.5 mg/kg）組、XFJNG（6 mg/kg，臨床等效劑量）組，同時(shí)設(shè)立對(duì)照組，每組10只，連續(xù)給藥4周。于給藥第0、1、2、3、4周進(jìn)行刻板運(yùn)動(dòng)評(píng)分；末次給藥后24 h殺鼠取材，試劑盒法檢測(cè)各組大鼠紋狀體內(nèi)多巴胺（DA）、去甲腎上腺素（NE）、5-羥色胺（5-HT）含量，下丘腦及血清促腎上腺皮質(zhì)素釋放激素（CRH）含量，血清促腎上腺皮質(zhì)激素（ACTH）、皮質(zhì)醇（CORT）含量；取腎上腺組織，進(jìn)行HE染色，光學(xué)顯微鏡下觀察大鼠腎上腺組織形態(tài)。結(jié)果 與模型組比較，給藥4周后，氟哌啶醇和XFJNG組大鼠刻板運(yùn)動(dòng)評(píng)分均顯著降低，紋狀體DA、NE、5-HT的水平均顯著升高，血清及下丘腦CRH水平均顯著降低，血清ACTH及CORT水平均顯著降低，差異均具有統(tǒng)計(jì)學(xué)意義（P<0.05）。與對(duì)照組比較，模型組大鼠腎上腺細(xì)胞體積較小，染色深，細(xì)胞質(zhì)占比較大，空泡明顯減少；氟哌啶醇、XFJNG組大鼠腎上腺細(xì)胞質(zhì)染色相對(duì)模型組較淺，空泡數(shù)量增多。結(jié)論 熄風(fēng)靜寧顆?？赡芡ㄟ^(guò)抑制大鼠紋狀體DA系統(tǒng)失衡及HPA軸功能亢進(jìn)來(lái)改善大鼠TS癥狀。

Objective To explore the effect of Xifeng Jingning Granule (XFJNG) on the HPA Axis and striatum monoamine neurotransmitters in tics Tourette syndrome (TS) rats.Methods TS model was induced by ig injection of iminodipropionitrile (IDPN, 150 mg/kg), then TS rats were randomly divided into model group, Haloperidol group (0.5 mg/kg), XFJNG group (6 mg/kg) and meanwhile establish control group, each group was 10 cases. Then the rats were treated for 4 weeks and the stereotyped movement scores were recorded in the 0, 1th, 2th, 3th, 4th week. The striatum levels of DA, NE, 5-HT, the hypothalamus and serum levels of CRH, the serum levels of ACTH and CORT were measured by kits. The adrenal gland tissues were stained with HE and the morphology of adrenal glands was observed under light microscope. Results Compared with the model group, the stereotyped movement scores of the rats in the medicine group and traditional medicine group were significantly decreased, the striatum levels of DA, NE, 5-HT, the hypothalamus and serum levels of CRH, the serum levels of ACTH and CORT in the medicine group and traditional medicine group were significantly decreased, all above had statistical difference (P<0.05). Compared with the control group, the adrenal cells in the model group were smaller, stained deeply, the proportion of cytoplasm was larger, and the number of vacuoles was significantly reduced. In the haloperidol group and XFJNG group, the adrenal gland cytoplasm staining was relatively shallow and the number of vacuoles increased. Conclusion XFJNG could improve TS by inhibiting DA system imbalance and HPA axis hyperfunction.

高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金項(xiàng)目（20120013120011）