目的 研究傣百解醇提物對(duì)非酒精性脂肪肝大鼠的治療作用，并探討其調(diào)控趨化素（Chemerin）及其受體（CMKLRI）表達(dá)的作用機(jī)制。方法 高脂飼料喂養(yǎng)8周建立非酒精性脂肪肝（NAFLD）大鼠模型，驗(yàn)證造模成功后改普通飼料喂養(yǎng)，并隨機(jī)分為模型組、非諾貝特膠囊組（陽性藥，0.2 g/kg）和傣百解醇提物低、高（2.6、4.5 g/kg）劑量組，每組10只，給藥組每天ig給藥1次，連續(xù)4周；對(duì)照組大鼠一直飼予普通飼料，對(duì)照、模型組給予等體積純水。最后1次給藥后大鼠禁食不禁水12 h，腹主動(dòng)脈取血收集血清，于全自動(dòng)生化分析儀上測(cè)定血清總膽固醇（TC）、三酰甘油（TG）、游離脂肪酸（FFA）、低密度脂蛋白膽固醇（LDL-C）、高密度脂蛋白膽固醇（HDL-C）、空腹血糖（FBG）水平；ELISA法檢測(cè)血清空腹胰島素（FINS）的濃度；計(jì)算胰島素敏感指數(shù)（ISI）和胰島素抵抗指數(shù)（HOMA-IR）。取肝組織進(jìn)行HE染色，光學(xué)顯微鏡下觀察肝組織病理學(xué)改變。實(shí)時(shí)熒光定量PCR（qRT-PCR）法檢測(cè)肝組織Chemerin及CMKLRI mRNA表達(dá)水平。結(jié)果 與模型組比較，傣百解醇提物高、低劑量組血清TC水平均顯著降低（P<0.05），低劑量組HDL-C含量顯著升高（P<0.05）；各劑量組HOMA-IR水平均降低，其中高劑量組差異顯著（P<0.05）；各劑量組ISI均有所升高，但作用均無統(tǒng)計(jì)學(xué)差異；高、低劑量組肝臟脂質(zhì)沉積等病理學(xué)改變有所改善；各劑量組Chemerin及CMKLRI mRNA表達(dá)均有所下調(diào)，其中高劑量組下調(diào)Chemerin mRNA表達(dá)作用顯著（P<0.05）。結(jié)論 傣百解醇提物對(duì)大鼠NAFLD具有降低LDL-C、升高HDL-C含量、降低HOMA-IR水平和保肝作用，其作用機(jī)制可能與下調(diào)chemerin mRNA表達(dá)有關(guān)。

Objective To study the therapeutic effect of ethanol extract of Dai Baijie (Ya Jie Xian Da) on non-alcoholic fatty liver rats, and to explore the mechanism of its regulation of Chemokine and its receptor (CMKLRI) expression. Method Non-alcoholic fatty liver (NAFLD) rat model was established by feeding high-fat diet for eight weeks. The rats were fed with normal diet after successful modeling. The rats were randomly divided into model group, fenofibrate capsule group (positive drug, 0.2 g/kg) and Dai Baijie alcohol extract low and high dose group (2.6 and 4.5 g/kg). Each group had 10 rats, and the treatment group was ig given once a day for four weeks, rats in control group were fed normal diet, and the control group and model group were given equal volume pure water. After fasting for 12 hours, blood samples were collected from abdominal aorta. Total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and fasting blood glucose (FBG) were measured by automatic biochemical analyzer. Levels of insulin, fasting insulin (FINS) and insulin sensitivity index (ISI) and insulin resistance index (HOMA-IR) were measured by ELISA. Liver tissues were stained with HE, and pathological changes of liver tissues were observed under light microscope. Real time fluorescence quantitative PCR (qRTPCR) was used to detect the expression level of Chemerin and CMKLRI mRNA in liver tissue. Results Comparison with the model group, after the intervention of Dai BaiJie (Ya Jie xian da) ethanol extract, The content of TC in serum in each dose group was decreased significantly (P<0.05), and the content of HDL-C in the low dose group was increased significantly (P<0.05). The levels of HOMA-IR in each dose group were decreased, and the difference was found in the high dose group; the ISI in each dose group was increased, but the effect was not statistically significant; the pathological changes such as liver lipid deposition were improved in the high and low dose groups; the expressions of Chemerin and CMKLRI mRNA in each dose group were downregulated, and the Chemerin mRNA expression down-regulated in the high dose group was remarkable. Conclusion Dai Baijie alcohol extract can reduce LDL-C, increase HDL-C content, decrease HOMA-IR level and protect liver in rats with NAFLD. The mechanism may be related to down-regulation of Chemerin mRNA expression.

云南省教育廳科學(xué)研究基金項(xiàng)目（2013Y234）