目的 考察玄歸滴丸對乙醇致大鼠急性胃潰瘍的保護(hù)作用并探討其作用機(jī)制。方法 65只SD大鼠隨機(jī)分為對照組、模型組、西咪替丁（50 mg/kg）組和玄歸滴丸低、中、高劑量（50、100、200 mg/kg，分別為臨床等效劑量的2、4、8倍）組。給藥組ig給予相應(yīng)的受試藥物，對照組及模型組ig相同劑量的生理鹽水，連續(xù)7 d，除了對照組外，其余各組均ig無水乙醇制備乙醇型胃潰瘍模型。造模1 h后處死大鼠，取胃組織進(jìn)行大體形態(tài)觀察，并進(jìn)行HE染色，光鏡下觀察病理形態(tài)變化；試劑盒法檢測胃組織中超氧化物歧化酶（SOD）、丙二醛（MDA）、髓過氧化物酶（MPO）、腫瘤壞死因子-α（TNF-α）、前列腺素E₂（PGE₂）和解痙多肽（SP）含量。結(jié)果 玄歸滴丸組的胃黏膜損傷程度、病理變化與模型組比較均有不同程度的減輕。與模型組比較，玄歸滴丸中劑量組SOD和低、中劑量組MPO水平均顯著升高（P<0.05、0.01）；中劑量組MDA水平顯著降低（P<0.05）；各劑量組TNF-α水平呈降低趨勢；低劑量組PGE₂含量顯著升高（P<0.05）；高劑量組SP含量顯著降低（P<0.05）。結(jié)論 玄歸滴丸對乙醇致急性胃潰瘍實(shí)驗(yàn)大鼠的胃黏膜損傷有較好的保護(hù)作用，其作用機(jī)制可能與抗氧化、抗炎作用有關(guān)。

Objective To investigate the protective effect of Xuangui Dropping Pills on alcohol-induced acute gastric ulcer in rats and explore its mechanism.Methods A total of 65 SD rats were randomly divided into control group, model group, cimetidine group (50 mg/kg) and Xuangui Dropping Pills low, medium and high dose (50, 100 and 200 mg/kg, 2, 4, 8 times of clinical equivalent dose, respectively) groups. The rats in the treatment group were given the corresponding drugs by gavage. The rats in control group and model group were fed with the same dose of saline for seven consecutive days. Except for control group, modeling of experimental gastric ulcer was carried out in all the other groups with anhydrous ethanol. Rats were executed one hour after establishment of model, and the gastric tissues were taken for gross morphological observation, HE staining and light microscopy were used to observe the pathological changes. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-alpha), prostaglandin E₂ (PGE₂) and spasmolytic polypeptide (SP) in gastric tissues were detected by kit method. Results Compared with model group, the degree of gastric mucosal injury and pathological changes in Xuangui Dropping Pills group were alleviated in varying degrees. Compared with model group, the SOD and MPO levels of Xuangui Dropping Pills in middle dose group increased significantly (P<0.05 and 0.01), while the MDA level in middle dose group decreased significantly (P<0.05). Compared with control group, the level of TNF-alpha in model group increased, and the content of TNF-α decreased after Xuangui Dropping Pill intervention. Compared with model group, the content of PGE₂ in Xuangui Dropping Pills low dose group was significantly higher (P<0.05), while the content of SP in high dose group was significantly lower (P<0.05). Conclusion Xuangui Dropping Pills can protect gastric mucosal injury induced by ethanol in rats with acute gastric ulcer. Its mechanism may be related to the regulation of SOD, MPO, SP, TNF-α and PGE₂ expression.

長江學(xué)者和創(chuàng)新團(tuán)隊(duì)發(fā)展計(jì)劃資助項(xiàng)目（IRT_14R41）