目的 采用實時熒光定量PCR （qRT-PCR）法和Illumina高通量測序技術研究杠柳毒苷和不同比例三七總皂苷配伍對大鼠腸道菌群的影響。方法 將15只SD大鼠隨機分為杠柳毒苷單獨給藥組（A組）、杠柳毒苷和低劑量三七總皂苷配伍組（B組）、杠柳毒苷和高劑量三七總皂苷配伍組（C組），每組5只。按杠柳毒苷10 mg/kg、三七總皂苷0.74和2.22 g/kg （分別相當于香加皮與三七的配伍比例為1：1和1：3）的劑量ig給藥，連續(xù)給藥7 d。給藥結束后收集大鼠糞便，進行qRT-PCR反應和高通量測序。結果 qRT-PCR結果表明，與杠柳毒苷單獨給藥組比較，杠柳毒苷和三七總皂苷配伍后，總菌、擬桿菌的相對含量顯著升高（P<0.05、0.01），而乳酸桿菌的相對含量呈現(xiàn)降低的趨勢；Illumina高通量測序結果顯示，在微生物群落結構上，杠柳毒苷配伍三七總皂苷后，擬桿菌的相對豐度明顯升高而乳酸桿菌的相對豐度呈現(xiàn)降低的趨勢；在腸道菌群多樣性上，3組樣品的多樣性指數(shù)間無顯著性差異。結論 杠柳毒苷和不同比例的三七總皂苷配伍后，可對大鼠腸道中的總菌、乳酸桿菌及擬桿菌產生一定影響，但對腸道菌群多樣性的影響不明顯。

Objective To study the influence of combination of Panax notoginseng saponins (PNS) and periplocin on intestinal flora in rats by using Illumina high-throughput sequencing and Real-time fluorescence quantitative PCR (qRT-PCR).Methods Fifteen SD rats were randomly divided into three groups:the periplocin single administration group (group A),compatibility group of periplocin and low dose PNS (group B),compatibility group of periplocin and high dose PNS (group C).Periplocin 10 mg/kg,PNS 0.74 and 2.22 g/kg (corresponding to the compatibility ratio of Periplocae Cortex and Panax notoginseng 1:1 and 1:3,respectively) were administered by ig for seven days.After seven days of administration,the fecal samples were collected and analyzed by qRT-PCR and Illumina high-throughput sequencing.Results qThe qRT-PCR results showed that the bacterium number of total gut bacteria and Bacteroidetes were significantly increased (P< 0.05 and 0.01),while the relative content of Lactobacillus was decreased in the combination group,compared with Periplocin group.Illumina high-throughput sequencing results showed that,in terms of microbial community structure,the relative abundance of Bacteroides was increased significantly and that of Lactobacillus was decreased after the combination of periplocin and PNS,and there was no significant difference in the diversity index of intestinal flora among three groups.Conclusion The compatibility of periplocin and PNS in different ratios has some effect on gut flora of rats to some extent,but has little effect on diversity of intestinal flora.

國家自然科學基金資助項目（81673824、81503457）