目的 探討益脈康片對放射性腦損傷小鼠的保護(hù)作用及主要作用機(jī)制。方法 將50只BALB/c小鼠隨機(jī)分為對照組、模型組和益脈康片低、中、高劑量（4.30、8.61、17.22 mg/kg）組，采用伽瑪?shù)秾Τ龑φ战M外其余各組小鼠進(jìn)行造模，連續(xù)7 d。造模結(jié)束后，益脈康片組ig給藥，對照組及模型組給予等量生理鹽水，連續(xù)給藥2周，1次/d。末次給藥24 h后，采用Morris法檢測各組小鼠的學(xué)習(xí)及記憶能力，并對各組小鼠海馬體CA1區(qū)進(jìn)行TUNEL染色及尼氏染色，采用免疫組化法檢測各組小鼠兔抗煙酰胺腺嘌呤二核苷酸磷酸酶氧化酶（NOX4）的表達(dá)情況，并對其進(jìn)行半定量分析。結(jié)果 第2~5天，對照組和益脈康片低、中、高劑量組潛伏期均明顯低于模型組（P<0.05）；與模型組比較，對照組和益脈康片低、中、高劑量組第Ⅲ象限時間及穿越平臺次數(shù)均明顯增加（P<0.05）；尼氏染色中，與模型組比較，益脈康片低、中、高劑量小鼠海馬體中神經(jīng)細(xì)胞核核仁不清晰及細(xì)胞形態(tài)異常程度均明顯減輕；TUNEL染色中，與模型組比較，益脈康片低、中、高劑量組均僅有呈散在的少量陽性染色神經(jīng)元；免疫組化中，與模型組比較，益脈康片低、中、高劑量組中NOX4陽性染色均明顯弱于模型組，且相對表達(dá)量均明顯低于模型組（P<0.05）。結(jié)論 益脈康片對放射性腦損傷小鼠具有較好的保護(hù)作用，其作用機(jī)制可能與有效抑制小鼠海馬體中NOX4表達(dá)有關(guān)。

Objective To study the protective effects and main mechanism of Yimaikang tablet on radiation-induced brain injury in mice.Methods A total of 50 BALB/c mice were divided into control group,model group,and Yimaikang tablet low,middle and high dose (4.30,8.61,and 17.22 mg/kg) treatment groups.The gamma knife was used to establish the radiation-induced brain injury model for continuous 7 d.After the modeling,mice in Yimaikang tablet group were ig administrated corresponding drugs,the other groups were treated by equivalence saline,lasted 2 weeks,once per day.24 hours after the last gavage,the Morris method was used to test the learning and memory ability of mice in each group.The TUNEL staining and Nissl's staining were performed on hippocampus CA1 region of mice.The immunohistochemical method was performed to observe the expression of NOX4 in mice,and the semi-quantitative analysis was also carried out.Results In the training of 2-5 d,the incubation periods of control group and low,middle,and high dose groups of Yimaikang tablet were significantly lower than model group (P< 0.05);Compared with the model group,the third quadrant residence time and times of crossing the platform in control group and Yimaikang tablet groups were significantly increased (P< 0.05).The TUNEL staining showed that there were small numbers of positive staining neurons in Yimaikang tablet groups when compared with the model group.The Nissl's staining showed that,compared with the model group,the cell morphological abnormalities and nucleoli blurring of neurocytes in the hippocampus of mice were significantly alleviated in low,middle,and high dose groups of Yimaikang tablet.From the results of immunohistochemical analysis,we found that the NOX4 positive stainings in Yimaikang tablet groups were significantly less than the model group,and the relative expression were significantly lower than model group (P< 0.05).Conclusion Yimaikang tablet has a good protective effects on radiation-induced brain injury in mice and the mechanism may be related to the effective inhibition of NOX4 expression in the hippocampus of mice.

陜西省中醫(yī)管理局中醫(yī)藥科研課題（15—LC064）