目的 研究牛樟芝水提物的抗過敏作用并初步探討其作用機制。方法 采用大鼠被動皮膚過敏模型、小鼠遲發(fā)型超敏模型、小鼠全身皮膚瘙癢模型、小鼠腹腔毛細血管通透性模型，觀察牛樟芝水提物在體抗過敏作用。采用體外培養(yǎng)的RBL-2H3肥大細胞，觀察牛樟芝水提物對細胞增殖及凋亡的影響，初步探討其抗過敏反應(yīng)的作用機制。結(jié)果 體內(nèi)試驗中，牛樟芝水提物顯著降低大鼠被動皮膚過敏反應(yīng)，明顯降低小鼠耳腫脹度、胸腺指數(shù)及脾指數(shù)，提高右旋糖酐所致皮膚瘙癢閾值，減少小鼠瘙癢次數(shù)，抑制組胺所致血管通透性上升；體外試驗中，其劑量相關(guān)性的抑制RBL-2H3細胞增殖，促進細胞凋亡。結(jié)論 牛樟芝水提取物有一定的抗過敏作用，其機制與促進RBL-2H3細胞凋亡有關(guān)。

Objective To explore the anti-allergic effects and possible mechanism of aqueous extract from Antrodia camphorata (EAC).Methods The anti-allergic effects of EAC were investigated in the passive cutaneous anaphylaxis (PCA) model of rat,in the delayed-type hypersensitivity (DTH) model of mice,in the dextran induced pruritus reaction model of mice,and in the peritoneal capillary permeability model of mice.RBL-2H3 mast cells were cultured in vitro to observe the effects of EAC on cell proliferation and apoptosis.Results EAC in vivo significantly reduced PCA reaction in rats,decreased the ear swelling degree,the index of thymus and spleen,and also significantly improved dextran-induced pruritic threshold and reduced the scratching number,and inhibited vascular permeability caused by histamine.EAC in vitro concentration-dependently inhibited RBL-2H3 mast cell proliferation and raised cell apoptosis level.Conclusion EAC has certain anti-allergic effects and its mechanism partially related to promote mast cell apoptosis.