目的 優(yōu)化大花紅景天再生體系并建立抗性篩選最佳條件，為建立大花紅景天高效遺傳轉(zhuǎn)化體系奠定基礎(chǔ)。方法 以大花紅景天葉片為外植體，觀察再生過程各階段在不同配比的6-BA、NAA、IBA誘導(dǎo)下的誘導(dǎo)率及生長狀況，并利用梯度篩選出外植體對卡那霉素（Kan）和抗潮霉素（Hyg）的抗性。結(jié)果 MS＋3.0 mg/L 6-BA＋1.0 mg/L NAA＋700 mg/L L-Pro為葉片不定芽分化的最佳培養(yǎng)基，分化率達(dá)到92%；MS＋700 mg/L L-Pro為根培養(yǎng)基；200 mg/L Kan，10 mg/L Hyg為大花紅景天遺傳轉(zhuǎn)化的最佳篩選壓；培養(yǎng)過程中添加10 mg/L Vc能有效抑制酚類物質(zhì)的外泌。結(jié)論 優(yōu)化了大花紅景天植株再生體系，篩選出適宜于大花紅景天遺傳轉(zhuǎn)化體系的Kan和Hyg篩選壓。

Objective To optimize the regeneration system of Rhodiola crenulata, to establish the optimal conditions for resistance screening, and to lay the foundation for the establishment of the efficient genetic transformation system of R. crenulata. Methods The leaves of Rhodiola crenulata were used as explants. The influences of different ratios of 6-BA, NAA, and IAA in the medium on callus induction and growth conditions were observed, and the implant resistance of Kan and Hyg was screened by gradient method. Results MS + 3.0 mg/L 6-BA + 1.0 mg/L NAA + 700 mg/L L-Pro was the optimal medium for the differentiation of leaf adventitious bud and the differentiation ratio of adventitious bud reached 92%; MS + 700 mg/L L-Pro was the media for adventitious roots; The best selection of genetic transformation system for R. crenulata were 200 mg/L Kan and 10 mg/L Hyg; Adding 10 mg/L Vc could effectively inhibit the phenolic substances secretion. Conclusion The regeneration system of R. crenulata, was optimized, and the pressure of Kan and Hyg for genetic transformation system of R. crenulata was screened.

國家科技支撐計(jì)劃（2011BA113B06），國家中醫(yī)藥管理局公共衛(wèi)生專項(xiàng)（20120716-540000）國家科技支撐計(jì)劃（2011BA113B06），國家中醫(yī)藥管理局公共衛(wèi)生專項(xiàng)（20120716-540000）