摘 要：目的 對24份不同產(chǎn)地石斛屬樣品的遺傳多樣性和親緣關(guān)系進(jìn)行分析。方法 采用ISSR分子標(biāo)記技術(shù)和非加權(quán)平均距離法（UPGMA）對24份石斛屬樣品進(jìn)行遺傳多樣性和聚類分析。結(jié)果 從100條ISSR引物中共篩選出6條多態(tài)性穩(wěn)定、清晰的引物， 24份石斛樣品共擴(kuò)增出847個DNA片段，平均每個引物擴(kuò)增出141個DNA片段，其多態(tài)性為100%。結(jié)論 24份不同產(chǎn)地石斛樣品被劃分為6個類群，遺傳多樣性非常豐富。

Abstract: Objective To analyze the genetic diversity and genetic relationship of 24 plant samples in Dendrobium SW. from different habitats. Methods The molecular marker technique of inter-simple sequence repeat (ISSR) and unweighted pair-group method with arithmetic means (UPGMA) were used to study the genetic diversity and cluster analysis of 24 samples. Results Six primers with stable and clear diversity were sieved from 100 ISSR primers. DNA fragments (847) were amplified from 24 samples, and 141 DNA fragments were amplified from each primer in average, with 100% of polymorphic bands. Conclusion The 24 samples in plants of Dendrobium SW. from different habitats are divided into six groups and the genetic diversity is very abundant.

農(nóng)業(yè)部熱帶作物種質(zhì)資源保護(hù)項目（12RZZY-39）；廣西科技攻關(guān)項目（桂科攻1222009-4）；廣西農(nóng)科院項目（201014，2011YZ11，2012GW03）