目的 解決南方紅豆杉生長緩慢的問題。方法 考察紅豆杉細胞懸浮培養(yǎng)過程中磷的消耗,并研究了碳、氮、磷對細胞生長及細胞活性的影響。結(jié)果 碳源在懸浮培養(yǎng)中期已經(jīng)消耗殆盡,后期碳源的缺乏限制了細胞的生長。結(jié)論 補加碳源組與對照組相比,細胞生長率和細胞密度明顯提高,其中細胞生長率約提高了83%。

Object To solve the problem that Taxus chinensis var. mairei (Lemee et Lévl.) Cheng et L. K.Fu grows slowly. Methods The consumption of phosphorus during the cell suspension culture and the effect of fed-batch carbohydrate, nitrogen and phosphorus on cell growth and living-cell activity were assayed. Results The carbohydrate was exhausted in the middle phase of suspension culture, dificiency of carbohydrate led to inhibition of cell growth in the late culture. Conclusion Compared with the control group, the cell growth rate and the cell density in the fed-batch carbohydrate group were increased significantly, and the cell growth rate was up to 83%.

天津市重點攻關項目(963107211);國家教委跨世紀優(yōu)秀人才培養(yǎng)計劃基金資助