目的 研究附子-甘草配伍前后湯液中沉積物的化學(xué)組成，以揭示附子-甘草配伍作用機(jī)制。方法 采用高效液相-飛行時(shí)間質(zhì)譜（HPLC-TOF-MS）對(duì)比分析附子-甘草合煎前后沉積物中6種酯型生物堿的量，借助傅里葉變換紅外光譜（FTIR）及二階光譜比較附子-甘草合煎前后沉積物的結(jié)構(gòu)變化。結(jié)果 附子配伍甘草合煎過程中，附子中大量酯型生物堿被甘草成分復(fù)合而發(fā)生沉積，沉積物產(chǎn)生的機(jī)制初步確定為生物堿中叔胺N與甘草羧酸C＝O發(fā)生締合。結(jié)論 初步探析了附子-甘草合煎前后湯液中沉積物化學(xué)組分變化，有助于揭示附子-甘草配伍過程中化學(xué)組分變化及配伍作用機(jī)制。

Abstract: Objective To study the chemical components in the sediments of Aconiti Lateralis Radix Praeparata (ALRP)- Glycyrrhizae Radix et Rhizoma (GRR) before and after their compatibility and to reveal the mechanism. Methods The contents of six ester alkaloids in the sediments of single ALRP and ALRP-GRR compatibility were compared simultaneously using HPLC-TOF-MS method, and the structural changes of the sediments in single ALRP and ALRP-GRR compatibility were identified using FTIR and second-derivative spectra. Results The sediments in ALRP-GRR compatibility would be produced by the association reaction between tertiary amine of alkaloids and carboxyl of compounds in GRR. Conclusion In this paper, the chemical information of the sediments in single ALRP and ALRP-GRR compatibility is preliminarily investigated, which would be beneficial to uncovering the material basis changes and compatibility mechanisms of ALRP and GRR.

國(guó)家自然科學(xué)基金面上項(xiàng)目（81073070）