目的 在不同海拔進(jìn)行當(dāng)歸Angelica sinensis生態(tài)適應(yīng)性實(shí)驗(yàn)，探索影響當(dāng)歸阿魏酸積累的關(guān)鍵因子。方法 通過田間實(shí)驗(yàn)測(cè)定當(dāng)歸阿魏酸量的變化和生理生化指標(biāo)、光合參數(shù)、生態(tài)因子。結(jié)果 當(dāng)歸根中阿魏酸量隨海拔升高而增加，且海拔2 780 m處理比海拔2 360 m處理高14.5%，差異顯著（P＜0.05）。分析影響當(dāng)歸阿魏酸積累的關(guān)鍵因子表明，降雨量（r＝0.898 8）和溫度（r＝?0.799 1）是關(guān)鍵生態(tài)因子，可溶性糖（r＝?0.974 9）和超氧化物歧化酶（SOD）（r＝?0.840 8）是關(guān)鍵生理生化因子，濕度（r＝0.969 9）和光合活性輻射(r＝0.946 7)是關(guān)鍵光合參數(shù)因子。結(jié)論 適當(dāng)升高種植海拔，增加降雨量和濕度，降低溫度和可溶性糖量均有利于當(dāng)歸中阿魏酸的轉(zhuǎn)化積累。

Abstract: Objective The ecological adaptability of Angelica sinensis was studied at different altitudes to explore the key factors on the accumulation of ferulic acid (FA) in A. sinensis. Methods Using the field test, the changes of physiological and biochemical indexes, photosynthetic parameters, ecological factors, and FA content were determined. Results FA content in the roots of A. sinensis was increased with the altitude rising, and the FA content in A. sinensis from 2 780 m was higher than that from 2 360 m by 14.5% (P < 0.05). The key factors that affected the accumulation of FA were analyzed; Rainfall (r = 0.898 8) and temperature (r = ?0.799 1) were the key ecological factors; Soluble sugar (r = ?0.974 9) and superoxide dismutase (SOD) (r = ?0.840 8) were the key physiological and biochemical factors; Relative humidity (r = 0.969 9) and photosynthetically active radiation (PAR) (r = 0.946 7) were the key photosynthetic factors. Conclusion Properly rising the altitude of planting, increasing the rainfall and humidity, and decreasing the temperature and soluble sugar are benefitial to the transformation and accumulation of FA in A. sinensis.

國家自然科學(xué)基金資助項(xiàng)目（310106182，31070352）