目的 探討全國范圍內(nèi)白花蛇舌草注射液物質(zhì)基礎(chǔ)的差異性。方法 采用超高效液相色譜-四級桿-飛行時間串聯(lián)質(zhì)譜（UPLC-QTOF-MS）對16批樣品進(jìn)行測定，對采集的數(shù)據(jù)以保留時間和質(zhì)荷比作為變量，進(jìn)行主成分分析（PCA）。結(jié)果 通過PCA發(fā)現(xiàn)不同企業(yè)生產(chǎn)的樣品自身差異較小，相互之間差異較大；通過載荷圖分析篩選出對分組影響比較大的7種標(biāo)記物。結(jié)論 全國范圍內(nèi)不同廠家生產(chǎn)的白花蛇舌草注射液不僅顏色差異較大，其內(nèi)在的物質(zhì)基礎(chǔ)也存在明顯的差異，這種差異主要體現(xiàn)在黃酮類和有機(jī)酸類成分的量上。

Objective To study the difference of substantial base of Baihuasheshecao (Hedyotis Diffusae Herba) Injection (BSCI) within the nationwide. Methods To determine 16 batches of samples by UPLC-QTOF-MS and analyze the data according to a variety of retention time and m/z by principal component analysis (PCA). Results The results showed that the difference itself was smaller but between each other was bigger among the different enterprises. Seven markers greatly impacted on the components through loading plot analysis were found. Conclusion BSCIs from different enterprises within nationwide are not only obviously different in color but also different in substantial base, which especially indicates the content of flavonoids and organic acids.

國家食品藥品監(jiān)督管理局項(xiàng)目（中2011003046001）