目的 明確大鼠ig給予逍遙散乙醇提取物的石油醚萃取物（XYP-A）后血清中移行成分的歸屬與結(jié)構(gòu)。方法 建立大鼠ig給予XYP-A后血清中移行成分的UPLC-PDA色譜分析方法。對全方石油醚萃取物及柴胡（紅柴胡）、當(dāng)歸、白術(shù)各單方石油醚萃取物分別ig給予大鼠后血清樣品色譜圖進(jìn)行比較，分析血清中移行成分的歸屬，通過與對照品比對，比較保留時(shí)間、UV掃描圖并結(jié)合文獻(xiàn)數(shù)據(jù)對其定性。結(jié)果 大鼠ig給予XYP-A后，血清中共檢測到20個(gè)入血成分，其中12個(gè)為原形成分，分別來源于柴胡9個(gè)、當(dāng)歸2個(gè)、白術(shù)1個(gè)，另外8個(gè)為代謝產(chǎn)物，并指認(rèn)了4個(gè)原形成分，分別為藁本內(nèi)酯、白術(shù)內(nèi)酯II、2, 8, 10-十五烷三烯-4, 6-二炔-1-醇和柴胡炔醇。結(jié)論 所建立的方法可行，血中檢測到的20個(gè)移行成分很可能是逍遙散體內(nèi)直接作用物質(zhì)，為闡明該方藥效物質(zhì)基礎(chǔ)提供了依據(jù)。

Objective To study the adscription and identification of transitional constituents in serum of rats after ig administration of petroleum ether fraction in ethanol extract from Xiaoyao Powder (XYP-A). Methods A UPLC-PDA method was established to identify the active components in serum of rats after ig administration of XYP-A and the single preparations of Bupleuri Radix (BR) Angelicae Sinensis Radix (ASR), and Atractylodis Macrocephalae Rhizoma (AMR). The transitional constituents were analyzed by comparing the fingerprints of the serum samples in formula, the single preparations and reference substances, referring literature, retention time, and UV scan spectra. Results Twenty components including 12 original components from XYP-A and eight metabolites were detected in serum of rats after ig administration of XYP-A. The original components consisted nine derived from BR, two from ASR, one from AMR, and the structures of ligustilide, atractylenolide II, 2, 8, 10-pentadecatriene-4, 6-diyne-1-ol (CH-1), and bupleurynol (CH-2) were identified. Conclusion The method is successfully applied for the serum pharmacochemistry study in rats. Twenty transitional constituents are absorbed into serum of rats and their metabolites may be the effective constituents of XYP-A acting directly to the body, which could lay the foundation for the serum pharmacodynamic study.

國家自然科學(xué)基金項(xiàng)目（81001688）；國際科技合作項(xiàng)目（2011DFA32630）；“重大新藥創(chuàng)制”科技重大專項(xiàng)（2012ZX09103201-035）；山西省基礎(chǔ)研究青年科技研究基金（2011021007-1）；山西省高等學(xué)校中青年拔尖創(chuàng)新人才支持計(jì)劃資助項(xiàng)目