目的 分析腐胺和過氧化氫調(diào)控白樺三萜合成中是否存在交互作用。方法 在白樺懸浮細胞的生長末期添加1 mmol/L腐胺（Put）、0.1 mmol/L過氧化氫（H₂O₂）和40 μg/mL真菌誘導(dǎo)子，采用比色法、高效液相色譜法和熒光顯微鏡分析白樺懸浮細胞中三萜量、多胺量和H₂O₂熒光強度變化。結(jié)果 1 mmol/L Put增加了細胞中H₂O₂的熒光強度和三萜量。0.1 mmol/L H₂O₂促進了三萜合成，卻抑制了多胺中亞精胺（Spd）、精胺（Spm）的合成，而對Put無影響。Put與H₂O₂同時處理組，細胞中H₂O₂的熒光強度和多胺的量均介于Put與H₂O₂單獨處理組之間，而三萜量卻顯著高于其單獨處理組；進一步藥理學(xué)實驗分析發(fā)現(xiàn)，真菌誘導(dǎo)子與H₂O₂清除劑過氧化氫酶（CAT）處理后，細胞中的Put和Spd量比真菌誘導(dǎo)后增加了0.62%和16.05%，而對Spm無顯著影響。真菌誘導(dǎo)子與多胺（Pas）合成抑制劑D-精氨酸（D-arg）處理組，細胞中的H₂O₂熒光強度比真菌誘導(dǎo)子處理組有所降低了；在真菌誘導(dǎo)子、CAT和D-arg同時處理下，細胞中的H₂O₂熒光強度、PAs量和三萜量均低于真菌誘導(dǎo)子與CAT或D-arg處理，但三萜量仍高于對照組。結(jié)論 Put與H₂O₂在促進白樺三萜合成中存在交互作用。

Objective To analyze the interaction between putrescine (Put) and hydrogen peroxide (H₂O₂) on the regulation of triterpenoid production in Betula platyphylla suspension cell culture. Methods Put (1 mmol/L), H₂O₂ (0.1 mmol/L), and fungal elicitors (40 μg/mL) were added into the eight-day-old B. platyphylla suspension cell culture, and the changes of triterpenoid content, polyamine content, and H₂O₂ fluorescence intensity were analyzed by chemical colorimetry, HPLC, and fluorescence microscopy, respectively. Results Put (1 mmol/L) increased the H₂O₂ fluorescence intensity and triterpenoid content in B. platyphylla suspension cell culture. H₂O₂ (0.1 mmol/L) promoted the triterpenoid production, inhibited the production of spermidine (Spd) and spermine (Spm), and had no effectt on Put content. Under the treatment of 1 mmol/L Put and 0.1 mmol/L H₂O₂, H₂O₂ fluorescence intensity and polyamine (PAs) content were between its separated treatment, and triterpenoid content was significantly higher than its separated treatment. Comparing with the fungal elicitor, fungal elicitor and H₂O₂ scavenger catalase (CAT) induced an increase of 0.62% and 16.05% in Put and Spd content, respectively, while no effect on Spm content. Fungal elicitor and PAs synthesis inhibitor D-arginine (D-arg) decreased the H₂O₂ fluorescence intensity. Under the treatment of fungal elicitor, the fluorescence intensity of CAT and D-arg, H₂O₂ and the contents of PAs and triterpenoid were lower than those of fungal elicitor and CAT or D-arg, but triterpenoid content was still higher than that of the control. Conclusion This can be inferred that the interaction between Put and H₂O₂ regulates the triterpenoid production in birch suspension cell culture.

中央高效基本科研業(yè)務(wù)專項資金項目（DL13EA08-03）；國家自然科學(xué)基金資助項目（31100445，31070531）；黑龍江省博士后科研啟動基金（LBH-Q11185）