目的 分析根腐病三七根內(nèi)細(xì)菌的多樣性。方法 用牛肉膏蛋白胨培養(yǎng)基分離根腐病三七根內(nèi)的細(xì)菌，經(jīng)細(xì)菌通用引物27F/1492R擴(kuò)增16S rDNA后，分別用Rsa I和Hin6 I限制性內(nèi)切酶對(duì)擴(kuò)增產(chǎn)物進(jìn)行酶切，結(jié)合限制性片段長度多態(tài)性（RFLP）分析方法和DNA測序技術(shù)，對(duì)分離自根腐病三七根內(nèi)的細(xì)菌進(jìn)行初步鑒定。結(jié)果 根腐病三七根內(nèi)的細(xì)菌分屬于8個(gè)類群，依占總菌數(shù)的比例分別是芽孢桿菌屬Bacillus 22.47%、無色桿菌屬Achromobacter 5.62%、寡養(yǎng)單胞菌屬Stenotrophomonas 5.62%、類芽孢桿菌屬Paenibacillus 4.49%、鞘氨醇桿菌屬Sphingobacterium 1.12%、蒼白桿菌屬Ochrobactrum 1.12%、不動(dòng)桿菌屬Acinetobacter 1.12%及腸桿菌科的一些屬58.43%。結(jié)論 泛菌屬和芽孢桿菌屬是根腐病三七根中的兩大優(yōu)勢(shì)細(xì)菌類群。

Objective To analyse the bacterial diversity in rotting roots of Panax notoginseng. Methods Bacterial strains were isolated from the diseased roots of P. notoginseng using beef extract-peptone medium. The 16S rDNA was amplificated by primer 27F/1492R, the product was digested by restriction endonuclease Rsa I and Hin6 I, and PCR-RFLP analysis and DNA sequencing technology were used to identify the bacterial strains in rotting roots of P. notoginseng. Results The bacteria could be divided into eight groups including Bacillus (22.47%), Paenibacillus (4.49%), Sphingobacterium (1.12%), Ochrobactrum (1.12%), Stenotrophomonas (5.62%), Achromobacter (5.62%), Acinetobacter (1.12%), and Enterobacteriaceae (58.43). Conclusion The dominant groups in the rotting roots of Panax notoginseng were identified as genera of Pantoea and Bacillus.

國家自然科學(xué)基金項(xiàng)目（30900963）；國家科技支撐項(xiàng)目（2011BAI13B01-02）；云南省中青年學(xué)術(shù)和技術(shù)帶頭人后備人才項(xiàng)目（2011CI027）；中國科學(xué)院“西部之光”人才培養(yǎng)計(jì)劃項(xiàng)目