目的 對(duì)菊科藥用植物菊Chrysanthemum morifolium非藥用部位化學(xué)成分的分布和動(dòng)態(tài)積累進(jìn)行分析評(píng)價(jià)，為該藥用生物資源的綜合利用提供科學(xué)依據(jù)。方法 分別采用超高效液相-三重四級(jí)桿質(zhì)譜聯(lián)用儀（UPLC-TQ/MS）、紫外可見(jiàn)分光光度法（UV）、超高效液相-二極管陣列檢測(cè)器（UPLC-DAD），測(cè)定不同生長(zhǎng)期菊根、莖、葉中氨基酸類、核苷類、黃酮類及有機(jī)酸類成分的存在及其量。結(jié)果 氨基酸類成分分析結(jié)果表明，菊的根、莖、葉中檢測(cè)到13種氨基酸，總氨基酸的量分布順序?yàn)椋焊救~＞莖；核苷類成分分析結(jié)果表明，菊葉中檢測(cè)到4種核苷，莖和根中分別檢測(cè)到2種核苷，總核苷的量分布順序?yàn)椋喝~＞根＞莖；黃酮類成分分析結(jié)果表明，總黃酮類成分的量分布順序?yàn)椋喝~＞根＞莖，其中葉片所含黃酮類成分量為9.94%～18.66%，根中質(zhì)量分?jǐn)?shù)為5.88%～8.02%，莖中質(zhì)量分?jǐn)?shù)為3.98%～5.41%；有機(jī)酸類成分分析表明，總有機(jī)酸的質(zhì)量分?jǐn)?shù)分布順序?yàn)椋喝~＞根＞莖，葉中質(zhì)量分?jǐn)?shù)為2.44%～4.94%，根中量為1.89%～2.64%，莖中質(zhì)量分?jǐn)?shù)為1.20%～1.48%。不同生長(zhǎng)期菊根、莖、葉中黃酮類和有機(jī)酸類成分量發(fā)生動(dòng)態(tài)變化，在菊花采摘后達(dá)到高峰。結(jié)論 菊非藥用部位尤其是葉中含有豐富的資源性化學(xué)成分，且在采摘花序后為資源豐產(chǎn)期。該研究結(jié)果為菊花采收后廢棄物的資源化利用提供了有益的借鑒。

Objective To analyze and evaluate the distribution and dynamic changes of chemical constituents from non-medicinal parts of Chrysanthemum morifolium, and to provide scientific basis for the comprehensive utilization of this medicinal biological resources Methods Ultra-high performance liquid chromatography combined with triple quadrupole electrospray tandem massspectrometry (UPLC-TQ/MS) was applied for measuring amino acids and nucleosides, UV spectrophotometry (UV) was used for determining flavones, and ultra-high performance liquid chromatography along with a diode array detector (UPLC-DAD) was used for analyzing flavones and organic acids of root, stem, and leaves of C. morifolium in different growing periods, respectively. Results There are 13 kinds of amino acids in the root, stem, and leaves, total amino acid content ranges of root > leaves > stem; there were four kinds of nucleosides in leaves while only tow kinds were detected in both root and stem. Total nucleoside content ranges of leaves > root > stem; content ranges of flavones in leaves, root and steam were 9.94%—18.66% in leaves; 5.88%—8.02% in root, and 3.98%—5.41% in stem, respectively. Content ranges of organic acids in leaves, root, and stem were 2.44%—4.94% in leaves, 1.89—2.64% in root, and 1.20%—1.48% in stem, respectively. The content changes of flavones and organic acids were the highest after the flowers were picked with growing. Conclusion There is abundant resources of chemical constituents in non-medicinal parts of C. morifolium,especially in leaves, and it reached the highest resource yield after picking the inflorescence. This research results has provided beneficial reference for the resource utilization of abandoned part of C. morifolium after picking the inflorescence.

江蘇高校中藥資源產(chǎn)業(yè)化過(guò)程協(xié)同創(chuàng)新中心建設(shè)項(xiàng)目（2013）