目的 為旋覆花屬8種藥用植物的分子鑒定提供依據(jù)。方法 本研究對(duì)4種旋覆花屬藥用植物的8個(gè)樣本ITS2序列進(jìn)行PCR擴(kuò)增和測(cè)序，同時(shí)從GenBank下載13個(gè)樣本。用MEGA5.0計(jì)算其種間、種內(nèi)的K2P距離，各序列變異位點(diǎn)并進(jìn)行聚類分析。結(jié)果 旋覆花屬8種藥用植物ITS2的長(zhǎng)度為210～212 bp，變異位點(diǎn)為60個(gè)；旋覆花藥材2種不同來(lái)源藥用植物有3個(gè)變異位點(diǎn)，與其他同屬6種植物有13～43個(gè)變異位點(diǎn)；構(gòu)建的系統(tǒng)發(fā)育樹(shù)顯示旋覆花藥材的不同基原樣本各聚為一支，能很好與其他6種植物區(qū)分；旋覆花Inula japonica、歐亞旋覆花I. britannica、總狀土木香I. racemosa、土木香I. helenium4個(gè)物種的遺傳距離值遠(yuǎn)小于與羊耳菊I. cappa、赤莖羊耳菊I. rubricaulis、顯脈旋覆花I. nervosa、澤蘭羊耳菊I. eupatoerioides 4個(gè)物種的遺傳距離值。結(jié)論 ITS2序列能夠?yàn)樾不▽?種藥用植物的鑒定和Flora of China對(duì)羊耳菊、赤莖羊耳菊、顯脈旋覆花、澤蘭羊耳菊分類修訂提供一定的分子證據(jù)。

Objective To provide evidence for the molecular identification of eight medicinal plants in Inula L. Methods Eight ITS2 sequence samples of four species in Inula L. were PCR amplified and sequenced, and 13 samples from the GenBank were downloaded. The intra-specific and inter-specific K-2P distances were calculated, and cluster analysis was constructed by MEGA 5.0. Results The length of ITS2 region varied from 210—212 bp with 60 variable sites. Inulae flos from two different sources have three variable sites, and its sibling species had 13 to 43 mutations; Different samples of Inula japonica and I. britannica were gathered together and could be distinguished from its sibling species by UPGMA tree. The genetic distances of four species between I. japonica, I. britannica, I. helenium, and I. racemosa were far less than those of four other species between I. cappa, I. rubricaulis, I.nervosa, and I.eupatoerioides. Conclusion The ITS2 sequence provides molecular evidence for the identification of eight species of medicinal plants in Inula L. and taxonomic revision of I. cappa, I. rubricaulis, I.nervosa, and I.eupatoerioides.

國(guó)家科技支撐計(jì)劃課題（2011BAI07B08）