目的 對(duì)栽培太子參的遺傳多樣性與藥材質(zhì)量進(jìn)行綜合評(píng)價(jià)，為合理利用太子參種質(zhì)資源及優(yōu)良品種選育提供依據(jù)。方法 采用ISSR分子標(biāo)記分析太子參12個(gè)栽培種源的遺傳多樣性，并用HPLC測(cè)定各種源藥材中太子參環(huán)肽B的量。結(jié)果 10條ISSR引物擴(kuò)增出82條帶，其中多態(tài)性條帶73條，多態(tài)位點(diǎn)百分率（PPL）為89.02%。Nei’s遺傳多樣性指數(shù)（H）平均值0.257 9，Shannon’s多態(tài)性指數(shù)（I）平均值為0.388 4，遺傳分化系數(shù)（Gst）為0.274 1，種源間基因流（Nm）為1.323 8。基于遺傳一致度，12個(gè)種源可聚為3類。太子參環(huán)肽B量在種源間和種源內(nèi)個(gè)體差異較大，種源4的太子參環(huán)肽B量（0.049 4%）明顯高于其他種源，且種源內(nèi)變異系數(shù)（9.51%）較小。結(jié)論 栽培太子參各產(chǎn)地間的換種及其生物學(xué)特性是其遺傳多樣性水平豐富的主要原因；綜合考慮遺傳多樣性水平和太子參環(huán)肽B量，種源3和4種質(zhì)資源優(yōu)良，適宜作為太子參種質(zhì)資源保存及優(yōu)良品種選育的對(duì)象。

Objective To study the genetic diversity and medicinal quality of Pesudostellaria heterophylla and to provide a reference for rational utilization on germplasm resources and fine variety breeding of P. heterophylla. Methods The genetic diversity of the 12 cultivated provenances of P. heterophylla were analyzed by ISSR molecular markers, and Pseudostellarin B in root of P. heterophylla were analyzed by HPLC. Results A total of 82 bands were produced by 10 primers, among which 73 bands were polymorphic bands, and the percentage of polymorphic bands (PPL) was 89.02%. The average value of Nei’s genetic diversity index (H) was 0.257 9, Shannon’s information index (I) was 0.388 4, genetic differentiation coefficient (Gst) was 0.274 1, and the gene flow (Nm) among provenances was 1.323 8. Cluster analysis based on genetic identity indicated that 12 provenances could be divided into three groups. There were great differences of Pseudostellarin B content among and within provenances. Pseudostellarin B content (0.049 4%) in provenance 4 was significantly higher than those in the others, and the coefficient of variation (9.51%) was less in this provenance. Conclusion The high genetic diversity could be attributed to the provenances exchange in different production areas of P. heterophylla and its biological characteristics. Comprehensive consideration of genetic diversity and Pseudostellarin B is made, and the provenances 3 and 4 of P. heterophylla have better quality, which are suitable for germplasm conservation and variety breeding.

施秉中藥材產(chǎn)業(yè)科技合作專項(xiàng)計(jì)劃 [施科合專項(xiàng)（2012）04號(hào)]；中央本級(jí)重大增減支項(xiàng)目（20603020223，20603020120）；貴陽市科技創(chuàng)新公共技術(shù)平臺(tái)項(xiàng)目（2010筑科合字第3-2號(hào)）