目的 建立紫背金盤Ajugae nipponensis愈傷組織誘導體系，篩選出具較高再生率的植株發(fā)生途徑，旨在構建高效、穩(wěn)定的再生體系。方法 MS為基本培養(yǎng)基，添加不同種類和濃度配比的植物生長調節(jié)劑，以莖尖、帶芽莖段和花序為外植體進行實驗。結果 花序是較適宜誘導愈傷組織的外植體，在MS＋6-BA 0.1 mg/L＋2, 4-D 1.5 mg/L中可在1周內誘導出愈傷組織，2周后即分化出綠色小芽叢；叢生芽增殖的適宜培養(yǎng)基為MS＋6-BA 2.0 mg/L＋NAA 0.5 mg/L，愈傷組織芽叢再生率高達100%，再生系數為4.10，4周后增殖倍數5.0以上；生根的適宜培養(yǎng)基為1/2 MS＋NAA 1.0 mg/L，3周后即可獲得再生植株，生根率100%；生根苗移栽至排水良好的沙土中，成活率100%。結論 紫背金盤不同外植體均可誘導出愈傷組織，其中花序愈傷發(fā)生率最好，是最適宜的外植體；本研究為保護紫背金盤野生資源和發(fā)展人工栽培奠定了良好基礎，也為遺傳轉化研究提供重要的科學依據。

Objective An efficient and stable regeneration system of Ajugae nipponensis was developed by establishing the callus induction system and selecting the best protocol for higher plant regeneration. Methods Stem tip, stem with buds, and inflorescence were all used as explants maintained on Murashige and Skoog (MS) medium supplemented with different types and concentration of plant growth regulator combinations. Results Inflorescence was the optimal explant for callus induction and was cultured on MS medium containing 0.1 mg/L 6-BA and 1.5 mg/L 2, 4-D in one week; The callus divided into green multiple shoot two weeks later; The optimal medium for multiple shoot proliferation was MS + 2.0 mg/L 6-BA + 0.5 mg/L NAA, the regeneration rate of callus clustered buds was as high as 100%, the regeneration coefficient was 4.10, and the proliferation increased by more than five times after four weeks; The optimal medium for rooting is 1/2 MS + 1.0 mg/L NAA, the plant regeneration occurred three weeks later and the rooting rate up to 100%; 100% of plantlet survived after transplanting rooted plantlets into sand. Conclusion The callus can be induced by all kinds of explants and inflorescence is the best explant for higher callus induction rate; The current study laid a solid foundation for protecting the wild resources and advancing the cultivated of A. nipponensis, meanwhile providing scientific evidence for the research of genetic transformation.

湖南省高?？萍紕?chuàng)新平臺資助項目（11K053）