目的 研究珠子參Panax japonicus var.major葉的化學(xué)成分。方法 采用硅膠、Sephadex LH-20、ODS柱色譜和半制備型高效液相色譜等方法進行分離純化，根據(jù)理化性質(zhì)和波譜數(shù)據(jù)分析鑒定化合物的結(jié)構(gòu)。結(jié)果 從珠子參中分離并鑒定了3個化合物，分別鑒定為20（21），24-達瑪二烯-3β，6α，12β-三醇（1）、20（22）Z，24-達瑪二烯-3β，6α，12β-三醇（2）、3-O-[β-D-吡喃葡萄糖-（1→2）-β-D-吡喃葡萄糖]-20-O-[-β-D-吡喃木糖-（1→6）-β-D-吡喃葡萄糖]-23E，25-二烯-20（S）-原人參二醇（3）。結(jié)論 化合物1和2為首次從該植物中分離得到，化合物3為新化合物，命名為珠子參苷Z。

Objective To study the chemical composition from the leaves of Panax japonicus var. major. Methods Column chromatographies (including macroporous resin, silica gel, Sephadex LH-20 and ODS) and semi-preparative HPLC were used to separate the constituents. The structures were elucidated by the analysis of spectral data and chemical properties. Results Three compounds were isolated and elucidated as dammar-20(21),24-diene-3β,6α,12β-triol (1), dammar-20(22) Z,24-diene-3β,6α,12β-triol (2), and 3-O-[-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl]-20-O-[-β-D-xylopyranosyl-(1→6)-β-D-glucopyranosyl]-23E,25-diene-20(S)-protopanoxadiol (3). Conclusion Compound 1 and 2 was obtained from the plant for the first time. And compound 3 named as majoroside Z is a new triterpenoid saponin.

陜西省教育廳服務(wù)地方專項計劃項目（14JF005）；陜西省科技廳項目（2017JM8087）；陜西省科技廳項目（2018ZCXM-SF-007）；陜西中醫(yī)藥大學(xué)學(xué)科創(chuàng)新團隊項目（2019-YL12）