[關(guān)鍵詞]
[摘要]
目的 研究不同形態(tài)梅花鹿鹿茸化學(xué)成分的差異。方法 采用紫外分光光度計(jì)、杜馬斯定氮儀、全自動(dòng)氨基酸分析儀、超高效液相色譜儀、高效液相色譜儀、氣相色譜儀、電感耦合等離子質(zhì)譜儀、原子熒光光度計(jì)等測(cè)定并比較了梅花鹿毛桃茸、二杠茸、三岔茸中多糖、粗蛋白、氨基酸、膠原蛋白、脂肪酸、礦質(zhì)元素、生物胺、核苷、硫酸軟骨素9類化學(xué)成分的含量差異,用主成分分析(PCA)法綜合評(píng)價(jià)不同形態(tài)梅花鹿鹿茸的質(zhì)量。結(jié)果 多糖、粗蛋白、氨基酸、膠原蛋白、脂肪酸、礦質(zhì)元素、生物胺、核苷、硫酸軟骨素在毛桃茸中質(zhì)量分?jǐn)?shù)分別為8.40 mg/g、44.82%、42.24%、23.23%、6 234.69 mg/kg、145.69 mg/g、55.12 mg/kg、2 271.87 mg/kg、0.74 mg/g;在二杠茸中質(zhì)量分?jǐn)?shù)分別為8.14 mg/g、52.12%、48.57%、21.50%、8 684.27 mg/kg、126.40 mg/g、76.14 mg/kg、3 438.37 mg/kg、1.94 mg/g;在三岔茸中質(zhì)量分?jǐn)?shù)分別為8.64 mg/g、51.86%、45.49%、22.31%、9 100.78 mg/kg、138.36 mg/g、70.75 mg/kg、2 507.82 mg/kg、1.84 mg/g。結(jié)論 不同形態(tài)梅花鹿鹿茸的化學(xué)成分有差異,PCA結(jié)果顯示,二杠茸質(zhì)量最好,三岔茸次之,毛桃茸質(zhì)量最差。該研究為不同形態(tài)鹿茸質(zhì)量評(píng)價(jià)及分等、分級(jí)標(biāo)準(zhǔn)提供參考。
[Key word]
[Abstract]
Objective In order to study the differences in chemical constituents of Cervi Cornu Pantotrichum in different forms of Cervus nipport. Methods The content of polysaccharides, crude protein, amino acids, collagen, fatty acids, mineral elements, biogenic amines, nucleosides, and chondroitin sulfate composition of Cervi Cornu Pantotrichum in different forms of C. nipport was determined and compared by using UV spectrophotometer, Dumas azotometer, automatic amino acid analyzer, UPLC, HPLC, gas chromatograph, inductively coupled plasma mass spectrometer and atomic fluorescence photometer. Principal component analysis was used to evaluate the quality of Cervi Cornu Pantotrichum. Results The content of polysaccharide, crude protein, amino acid, collagen, fatty acid, mineral element, biogenic amine, nucleoside and chondroitin sulfate in one-branched velvet antler was 8.40 mg/g, 44.82%, 42.24%, 23.23%, 6 234.69 mg/kg, 145.69 mg/g, 55.12 mg/kg, 2 271.87 mg/kg, and 0.74 mg/g, respectively; The content in two-branched velvet antler was 8.14 mg/g, 52.12%, 48.57%, 21.50%, 8 684.27 mg/kg, 126.40 mg/g, 76.14 mg/kg, 3 438.37 mg/kg, and 1.94 mg/g, respectively; The content in three-branched velvet antler was 8.64 mg/g, 51.86%, 45.49%, 22.31%, 9 100.78 mg/kg, 138.36 mg/g, 70.75 mg/kg, 2 507.82 mg/kg, and 1.84 mg/g, respectively. Conclusion The chemical composition of Cervi Cornu Pantotrichum in different forms of C. nipport was different. The results of principal component analysis showed that the quality of the two-branched velvet antler was the best, the three-branched velvet antler was the second, the one-branched velvet antler was the worst. This study provides a reference for the quality evaluation and grading standards of different forms of Cervi Cornu Pantotrichum.
[中圖分類號(hào)]
[基金項(xiàng)目]
國(guó)家重點(diǎn)研發(fā)計(jì)劃(2018YFC1706604);國(guó)家重點(diǎn)研發(fā)計(jì)劃(2018YFC1706605);中國(guó)農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程項(xiàng)目(CAAS-ASTIP-2016-ISAPS);吉林省科技發(fā)展計(jì)劃項(xiàng)目(20180201076YY);吉林省科技發(fā)展計(jì)劃項(xiàng)目(20170309002YY)