目的 基于HPLC建立甘草指紋圖譜及同時測定甘草中6個成分含量的分析方法，并考察4種軟化方式（淋潤、常壓蒸潤、70℃減壓蒸潤與85℃減壓蒸潤）對甘草質(zhì)量的影響。方法 采用紫外分光光度法，以甘草苷、甘草酸為對照品，測定甘草中總黃酮及總皂苷的含量；基于HPLC同時測定甘草中甘草苷、芒柄花苷、異甘草苷、甘草素、刺甘草查耳酮、甘草酸6個成分的含量，比較了不同軟化方法處理后的甘草中指標(biāo)成分含量的變化；利用中藥色譜指紋圖譜相似度評價系統(tǒng)對不同軟化處理后的甘草樣品進(jìn)行相似度評價，并進(jìn)行聚類分析。結(jié)果 總黃酮、總皂苷的結(jié)果顯示，未加工過的甘草中總黃酮與總皂苷的含量最高，淋潤的甘草總黃酮與總皂苷含量偏低。常壓蒸潤、70℃減壓蒸潤和85℃減壓蒸潤這3種處理方法對甘草質(zhì)量影響不大；含量測定結(jié)果顯示，不同軟化處理后，異甘草苷的含量均顯著降低，而不同的軟化處理組之間，所有成分含量差異不顯著。指紋圖譜相似性評價與聚類分析表明，3批甘草不同軟化處理方法后的樣品與未加工組分別聚為一類，而不同軟化處理方法對于甘草整體化學(xué)成分組成無顯著差異。結(jié)論 建立的分析方法，能夠快速、準(zhǔn)確地同時測定甘草中的6種成分，而且尤其需要對軟化過程中異甘草苷的含量進(jìn)行監(jiān)測。綜合生產(chǎn)成本、生產(chǎn)效率及不同指標(biāo)的質(zhì)量評價結(jié)果，常壓蒸潤在生產(chǎn)過程中可行性最高。本研究為甘草軟化的大生產(chǎn)提供了理論指導(dǎo)，有利于進(jìn)一步規(guī)范甘草飲片的生產(chǎn)流程。

Objective A method was established to obtain fingerprint and determination of six components in Glycyrrhizae Radix et Rhizoma Pieces (GRRP) based on HPLC-PDA, and samples with four kinds of softening methods (showering moistening, steaming, 70℃ decompression steaming, 85℃ decompression steaming) were analyzed. Methods The content of total flavonoids and total saponins was determined by ultraviolet spectrophotometry with liquiritin and glycyrrhizic acid as reference materials. Simultaneous determination of six components of liquiritin, ononin, isoliquiritin, glycyrrhizin, echinatin, glycyrrhizic acid was performed based on HPLC. Changes of the components content in the samples which treated by different softening methods were compared. The similarity evaluation of samples with different softening methods was carried out by the chromatographic fingerprint similarity evaluation system of traditional Chinese medicine, and cluster analysis was also carried out. Results The results showed that the content of total flavonoids and total saponins in untreated samples was the highest, and the content of total flavonoids and total saponins in samples treated by showering moistening was the lowest. The three treatment methods of atmospheric pressure steaming, steaming decompression at 70℃ and steaming decompression at 85℃ had little effect on the samples. The content determination showed that the content of isoliquiritin was decreased significantly after softening treatment. The difference among the different softening treatment groups was not significant. The samples with different softening methods of the three batches of samples were grouped together with their raw products. Different softening methods had no significant difference in the composition of the medicinal herbs. Conclusion The established method can quickly and accurately determine the six components, and in particular, the content of isoglycyrrhizin should be monitored. Combining production efficiency, production cost and quality evaluation, steaming is the most feasible in the production process. This study provided theoretical guidance for the large-scale production of softening, which was conducive to further standardizing the production process of GRRP.

“國家重點研發(fā)計劃”資助項目（2018YFC1706500）；“國家重點研發(fā)計劃”資助項目（2018YFC1707000）