目的 探討甘草總皂苷及水提物對(duì)肝損傷大鼠腸道菌群的影響。方法 采用ip四氯化碳誘導(dǎo)大鼠慢性肝損傷模型，模型大鼠分為4組，分別ig給予甘草總皂苷和甘草水提物，并以聯(lián)苯雙酯作為陽性對(duì)照組，模型組ig生理鹽水。同時(shí)設(shè)對(duì)照組，ig給予生理鹽水。于給藥結(jié)束后，測定各組大鼠血清中丙氨酸轉(zhuǎn)氨酶（ALT）、天冬氨酸轉(zhuǎn)氨酶（AST）水平；收集大鼠糞便，通過16 S rRNA測序?qū)Ω鹘M大鼠腸道菌群的多樣性進(jìn)行分析。結(jié)果 與對(duì)照組比較，模型組大鼠血清中ALT、AST水平顯著升高（P<0.05、0.01）；與模型組比較，各給藥組大鼠血清中ALT、AST水平均顯著降低（P<0.05、0.01）；與模型組比較，聯(lián)苯雙酯組大鼠菌群結(jié)構(gòu)未發(fā)生顯著變化，而甘草總皂苷組及甘草水提物組均表現(xiàn)出不同的群落組成。并且相對(duì)于水提物組，總皂苷組顯著提高了乳酸桿菌屬和桿菌門S24-7科菌群的相對(duì)豐度。但甘草總皂苷組和甘草水提物組的大鼠腸道菌群與對(duì)照組大鼠腸道菌群之間仍存在差異，并沒有完全恢復(fù)到正常狀態(tài)。結(jié)論 甘草總皂苷與甘草水提物均可以改善慢性肝損傷，并對(duì)肝損傷大鼠腸道菌群發(fā)揮不同的調(diào)控作用。

Objective To explore the effect of total saponins of Glycyrrhiza inflata and its decoction on intestinal flora in rats with liver injury. Methods Model of rats with liver injury was induced by intraperitoneal injection of carbon tetrachloride, which were randomly divided into four groups, including total saponins group (Sap group), water decoction group (Dec group), bifendate group (Bif group) and physiological saline group (NS group), then they were given drugs by oral admission. At the same time, a blank control was used (Control group), and the rats were given physiological saline by oral admission. Finally, the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum of each group of rats were measured; The rat feces were collected for 16 S rRNA sequencing of intestinal flora. Results Compared with the control group, the levels of ALT and AST in the serum of the model group were significantly increased (P<0.05, P<0.01). Compared with the model group, the levels of ALT and AST in the serum of the rats in each administration group were significantly reduced (P<0.05, P<0.01). Compared with the NS group, the intestinal flora in Bif group did not change significantly, while the Sap group and the Dec group showed different community composition. Total saponins significantly increased the relative abundance of Lactobacillus and Bacteroidales_S24-7 compared with water decoction. However, the intestinal flora of the Sap group and the Dec group were still different from the intestinal flora of the control group, and did not completely return to the normal state. Conclusion Total saponins of G. inflata and its decoction may play different roles in regulating the composition of intestinal flora in rats with liver injury, and then improve the pathological condition of liver injury.

江蘇省研究生科研與實(shí)踐創(chuàng)新計(jì)劃項(xiàng)目（KYCX18_1624）