目的 比較2種虎耳草科植物楔葉茶藨（RDP）和東北茶藨子（RMK）抗腎纖維化的藥效作用差異。方法 采用體外實驗與體內(nèi)實驗相結(jié)合的方法，利用轉(zhuǎn)化生長因子-β1（TGF-β1）誘導(dǎo)的人腎小管上皮細(xì)胞（HK-2）纖維化模型和單側(cè)輸尿管結(jié)扎（UUO）誘導(dǎo)的小鼠腎纖維化模型，綜合運用HE染色、Masson染色，免疫組化，免疫印跡等方法，以細(xì)胞形態(tài)、細(xì)胞長度與寬度比值，腎臟組織病理損傷程度，腎臟組織中E-鈣黏蛋白（E-cadherin）和α-平滑肌肌動蛋白（α-SMA）的蛋白表達(dá)等為主要觀測指標(biāo)，比較RDP和RMK提取物對各項指標(biāo)的影響；采用偏最小二乘-判別分析（PLS-DA）法，對RDP和RMK治療組小鼠腎臟組織病理學(xué)評分進行分析。結(jié)果 RDP提取物（1.5、5、15μg/mL）與RMK提取物（3、10 μg/mL）均可以有效改善TGF-β1誘導(dǎo)的HK-2細(xì)胞形態(tài)改變，減小細(xì)胞長度與寬度比值；RDP（40 mg/kg）與RMK（80 mg/kg）均能顯著減少UUO模型小鼠α-SMA表達(dá)和增加E-cadherin的蛋白表達(dá)，并減輕病理性損傷和纖維化程度；PLS-DA分析顯示，RDP和RMK改善UUO誘導(dǎo)腎臟組織纖維化的作用無明顯差異。結(jié)論 RDP和RMK體內(nèi)外對腎纖維化均具有一定的保護作用，RMK和RDP對各項指標(biāo)的改善作用無明顯差別。

Objective To compare pharmacodynamic difference of Ribes diacanthum (RDP) and Ribes mandshuricum (RMK) treatment on renal fibrosis in vivo and in vitro. Methods Both of TGFβ1-induced HK-2 cell fibrosis model and UUO-induced kidney fibrosis mice model were used in the present study. The cell morphology, ratio of cell length to width, renal histopathology, protein expressions of α-SMA and E-cadherin in kidney tissues were evaluated through biological and pharmacological methods and technologies, including Western blot, immunohistochemistry, HE staining, Masson staining and so on. In addition, partial least squares-discriminant analysis (PLS-DA) was applied to analyze the renal histopathological score as well. Results RDP (1.5, 5, 15 μg/mL) and RMK (3, 10 μg/mL) effectively improved morphological changes and reduced the ratio of cell length to width in TGFβ1-induced HK-2 cell fibrosis; Moreover, RDP (40 mg/kg) and RMK (80 mg/kg) remarkably decreased the expression of α-SMA and increased the expression of E-cadherin in UUO mice model. The degree of pathological damage and fibrosis were also alleviated in both groups. PLS-DA analysis showed no significant difference in anti-fibrotic effects between RDP and RMK treatment. Conclusion Both RDP and RMK have anti-fibrosis effects on TGFβ1-induced HK-2 cell fibrosis model and UUO-induced kidney fibrosis mice model, and there is no significant difference between these two herbs.

楔葉茶藨腎保護活性物質(zhì)基礎(chǔ)及作用機制研究（81603389）；江蘇省自然科學(xué)基金面上項目：基于轉(zhuǎn)化醫(yī)學(xué)研究楔葉茶藨腎保護作用的物質(zhì)基礎(chǔ)及作用機制研究（BK20160754）